Linked Life Data

9597772

Source:http://linkedlifedata.com/resource/pubmed/id/9597772

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-6-15
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF017371, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF017372, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF017373, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF017374, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF017375, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF017376, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF017377, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF017378, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF017379, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF017380, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF017381, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF017382, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF031154, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF031155, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF031156, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF031157, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF031158
pubmed:abstractText
A tandemly repeated satellite DNA of 290-291 base pairs (bp) was identified by SalI digestion of genomic DNA of five species of Eastern Pacific (California) abalone (genus, Haliotis). Following cloning and sequencing of one repeat unit from one species, the consensus sequences of this satellite were determined for five species by directly sequencing genomic DNA using satellite-specific primers. Phylogenetic trees of the consensus satellite sequences had the same topology as trees constructed for two abalone sperm acrosomal proteins. In 12 randomly picked clones of the Red abalone (H. rufescens) SalI satellite, 16 positions varied, the variation being spread throughout the sequence. GenBank database searches found no significant similarities between this satellite and known sequences. Southern analysis showed that all 290-bp SalI repeats were excised from genomic DNA by Sau3A1 digestion. The tandem arrangement of satellite repeats was confirmed by sequencing through the SalI site into the next repeat using genomic DNA as template, time-dependent appearance of DNA ladders with an approximate 300-bp spacing in SalI digests of genomic DNA, and ladders of bands with an approximate 300-bp spacing generated by polymerase chain reaction (PCR) using genomic DNA as template. In the Red abalone, the 290-bp SalI satellite represents approximately 0.5% of total DNA, equivalent to approximately 28,000 copies per haploid genome. The species-specific consensus sequence of this satellite, obtained directly using genomic DNA as the sequencing template, provides a molecular marker that could be used for identification of hybrid parentage, taxonomy, population identification, and forensic studies.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1053-6426
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Direct sequencing of genomic DNA for characterization of a satellite DNA in five species of eastern Pacific abalone.
pubmed:affiliation
Center for Marine Biotechnology and Biomedicine, University of California, San Diego, La Jolla 92093-0202, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.