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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1998-8-25
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pubmed:abstractText |
Antisera raised against galectin-1 exhibit crossreactivities with other galectins or related molecules. In order to overcome this problem, a monoclonal antibody to human brain galectin-1 was obtained by selecting clones without reactivity toward galectin-3. This mAb specifically bound galectin-1 of various animal origins but neither galectin-2 nor galectin-3. Western-blotting analysis of soluble human brain extracts after 2D gel electrophoresis revealed only the two most acidic isoforms of galectin-1. The ability of this mAb to bind galectin-1/asialofetuin complexes indicates that its epitope is not localized in the carbohydrate recognition domain of galectin-1. This particularity induces with efficiency its monospecificity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0959-6658
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
425-32
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:9597540-Amino Acid Sequence,
pubmed-meshheading:9597540-Antibodies, Monoclonal,
pubmed-meshheading:9597540-Antibody Specificity,
pubmed-meshheading:9597540-Antigens, Differentiation,
pubmed-meshheading:9597540-Brain Chemistry,
pubmed-meshheading:9597540-Cross Reactions,
pubmed-meshheading:9597540-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:9597540-Galectin 1,
pubmed-meshheading:9597540-Galectin 2,
pubmed-meshheading:9597540-Galectin 3,
pubmed-meshheading:9597540-Hemagglutinins,
pubmed-meshheading:9597540-Humans,
pubmed-meshheading:9597540-Lectins,
pubmed-meshheading:9597540-Molecular Sequence Data,
pubmed-meshheading:9597540-Sequence Alignment,
pubmed-meshheading:9597540-Sequence Homology, Amino Acid
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pubmed:year |
1998
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pubmed:articleTitle |
Production and characterization of a monoclonal antibody able to discriminate galectin-1 from galectin-2 and galectin-3.
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pubmed:affiliation |
Laboratoire de Biochimie et Technologie des Protéines (EA 1625), Université Paris Nord, UFR Santé, Médecine et Biologie Humaine, Bobigny, France.
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pubmed:publicationType |
Journal Article
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