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rdf:type |
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|
lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1998-7-29
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|
pubmed:abstractText |
Treatment of rabbit brain membranes of the DHP binding sites of L-type Ca2+ channel with lysine-specific reagent resulted in a time- and concentration-dependent loss of [3H]nitrendipine binding activity. Following exposure to the maximum concentration of PLP (100 mM), [3H]nitrendipine binding was inhibited by up to 96.5%. Scatchard analysis of the binding data indicated that treatment with PLP resulted in a loss of [3H]nitrendipine binding sites with no effect on binding affinity. Considerable protection against PLP inactivation was obtained by nifedipine. These results indicate that lysine residue plays a critical role in maintaining the DHP-binding sites in a conformation capable of ligand binding.
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pubmed:language |
eng
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pubmed:journal |
|
|
pubmed:citationSubset |
IM
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pubmed:chemical |
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|
pubmed:status |
MEDLINE
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|
pubmed:month |
Apr
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|
pubmed:issn |
0197-0186
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
32
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pubmed:owner |
NLM
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|
pubmed:authorsComplete |
Y
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pubmed:pagination |
361-4
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|
pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
|
|
pubmed:year |
1998
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|
pubmed:articleTitle |
Chemical modification of the dihydropyridines binding sites by lysine reagent, pyridoxal 5'-phosphate.
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|
pubmed:affiliation |
Dipartimento di Psichiatria, Neurobiologia, Farmacologia e Biotecnologie, Università degli Studi di Pisa, Italy.
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pubmed:publicationType |
Journal Article
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