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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1998-6-12
|
| pubmed:abstractText |
Plasmid pTO1 containing the oriT fragment from RK2, the Escherichia coli replication function from pBR322, and a DNA fragment of actinophage phi C31 with the attachment site was transferred from E. coli S17-1 to strains of the genera Actinomadura, Arthrobacter, Micromonospora, Nocardia, Rhodococcus, and to 16 strains of the genus Streptomyces. The frequency of conjugant formation was 1 x 10(-3)-1 x 10(-5) depending on the strain. Hybridization experiments demonstrated that plasmid pTO1 integrates into chromosomes of a number of the recipient strains examined.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0378-1097
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
162
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
47-52
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9595662-Actinomycetales,
pubmed-meshheading:9595662-Attachment Sites, Microbiological,
pubmed-meshheading:9595662-Bacteriophages,
pubmed-meshheading:9595662-Chromosomes, Bacterial,
pubmed-meshheading:9595662-Conjugation, Genetic,
pubmed-meshheading:9595662-Escherichia coli,
pubmed-meshheading:9595662-Plasmids,
pubmed-meshheading:9595662-Streptomyces
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Transfer of plasmid pTO1 from Escherichia coli to various representatives of the order Actinomycetales by intergeneric conjugation.
|
| pubmed:affiliation |
State Scientific Centre of the Russian Federation, GNIIGENETIKA, Moscow, Russia. LOMOV@VNIGEN.MSK.SU
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|