| pubmed-article:9594023 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9594023 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:9594023 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:9594023 | lifeskim:mentions | umls-concept:C0677626 | lld:lifeskim |
| pubmed-article:9594023 | lifeskim:mentions | umls-concept:C1456820 | lld:lifeskim |
| pubmed-article:9594023 | lifeskim:mentions | umls-concept:C1521761 | lld:lifeskim |
| pubmed-article:9594023 | lifeskim:mentions | umls-concept:C1948023 | lld:lifeskim |
| pubmed-article:9594023 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:9594023 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:9594023 | pubmed:dateCreated | 1998-7-17 | lld:pubmed |
| pubmed-article:9594023 | pubmed:abstractText | The syndrome of cachexia associated with malignant diseases can be in part attributed to the effects of tumour necrosis factor alpha (TNFalpha) which itself is produced by a variety of tumour cells. We have recently reported that the human hepatoma cell line HepG2 expresses the TNFalpha gene and releases biologically active TNFalpha protein after stimulation with interleukin-1beta (IL-1beta). Granulocyte-colony stimulating factor (G-CSF) is a glycoprotein necessary for the proliferation and differentiation of neutrophil progenitor cells in the bone marrow. In addition G-CSF has been reported to exert anti-inflammatory effects. In our study we tested the effect of recombinant human G-CSF (rhG-CSF) on TNFalpha production in HepG2 cells. It could be shown that rhG-CSF (250 U/ml) significantly reduced IL-1beta-induced (300 pg/ml) TNFalpha gene expression after 1-h and 3-h incubation periods (TNFalpha mRNA concentrations were: 8.8+/-2.1 amol/ microg total RNA after a 1-h incubation with IL-1beta versus 3.8+/-1.3 amol/ microg total RNA after a 1-h incubation with IL-1beta + rhG-CSF and 13.8+/-2.2 amol/ microg total RNA after a 3-h incubation with IL-1beta versus 8.8+/-2. 1 amol/ microg total RNA after a 3-h incubation with IL-1beta + rhG-CSF). From these data we conclude that rhG-CSF is a potent inhibitor of cytokine-induced TNFalpha production by tumour cells. Therefore, treatment of patients with malignant diseases with rhG-CSF might represent a useful tool to improve the tumour-associated cachexia. | lld:pubmed |
| pubmed-article:9594023 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9594023 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9594023 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9594023 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9594023 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9594023 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9594023 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9594023 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9594023 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9594023 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9594023 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:9594023 | pubmed:issn | 0031-6768 | lld:pubmed |
| pubmed-article:9594023 | pubmed:author | pubmed-author:HoffmannGG | lld:pubmed |
| pubmed-article:9594023 | pubmed:author | pubmed-author:Schobersberge... | lld:pubmed |
| pubmed-article:9594023 | pubmed:author | pubmed-author:FredeSS | lld:pubmed |
| pubmed-article:9594023 | pubmed:author | pubmed-author:DeetjenCC | lld:pubmed |
| pubmed-article:9594023 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9594023 | pubmed:volume | 436 | lld:pubmed |
| pubmed-article:9594023 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9594023 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9594023 | pubmed:pagination | 233-7 | lld:pubmed |
| pubmed-article:9594023 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:9594023 | pubmed:meshHeading | pubmed-meshheading:9594023-... | lld:pubmed |
| pubmed-article:9594023 | pubmed:meshHeading | pubmed-meshheading:9594023-... | lld:pubmed |
| pubmed-article:9594023 | pubmed:meshHeading | pubmed-meshheading:9594023-... | lld:pubmed |
| pubmed-article:9594023 | pubmed:meshHeading | pubmed-meshheading:9594023-... | lld:pubmed |
| pubmed-article:9594023 | pubmed:meshHeading | pubmed-meshheading:9594023-... | lld:pubmed |
| pubmed-article:9594023 | pubmed:meshHeading | pubmed-meshheading:9594023-... | lld:pubmed |
| pubmed-article:9594023 | pubmed:meshHeading | pubmed-meshheading:9594023-... | lld:pubmed |
| pubmed-article:9594023 | pubmed:meshHeading | pubmed-meshheading:9594023-... | lld:pubmed |
| pubmed-article:9594023 | pubmed:meshHeading | pubmed-meshheading:9594023-... | lld:pubmed |
| pubmed-article:9594023 | pubmed:meshHeading | pubmed-meshheading:9594023-... | lld:pubmed |
| pubmed-article:9594023 | pubmed:meshHeading | pubmed-meshheading:9594023-... | lld:pubmed |
| pubmed-article:9594023 | pubmed:meshHeading | pubmed-meshheading:9594023-... | lld:pubmed |
| pubmed-article:9594023 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9594023 | pubmed:articleTitle | Granulocyte-colony stimulating factor inhibits TNFalpha production in a human hepatoma cell line. | lld:pubmed |
| pubmed-article:9594023 | pubmed:affiliation | Department of Physiology I, University of Bonn, Nussallee 11, D-53115 Bonn, Germany. | lld:pubmed |
| pubmed-article:9594023 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9594023 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
