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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1998-7-31
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pubmed:abstractText |
Advanced glycation endproducts have been implicated in a number of diabetic and aging changes. Some of these effects occur in part through induction of cytokines such as platelet-derived growth factor (PDGF), which is expressed by the retinal pigment epithelium (RPE). In this study, cultures of RPE were evaluated for PDGF expression after treatment with pentosidine, a well characterized advanced glycation endproduct. Northern analysis provided evidence for the increased expression of a 3.7 kb PDGF-B transcript over unstimulated controls in the established ARPE-19 cell line. Western analysis demonstrated increased PDGF-BB protein in conditioned medium compared to controls of ARPE-19 cells. In addition, two different early passage cultures of RPE showed increased PDGF-BB protein after pentosidine treatment compared to unstimulated controls. The enhanced production of PDGF-BB could play a role in the maintenance of the RPE-Bruch's membrane complex and influence changes associated with diabetes and aging.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anticoagulants,
http://linkedlifedata.com/resource/pubmed/chemical/Arginine,
http://linkedlifedata.com/resource/pubmed/chemical/Lysine,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet-Derived Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/pentosidine,
http://linkedlifedata.com/resource/pubmed/chemical/platelet-derived growth factor BB
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0014-4835
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 1998 Academic Press Limited.
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pubmed:issnType |
Print
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pubmed:volume |
66
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
411-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9593635-Anticoagulants,
pubmed-meshheading:9593635-Arginine,
pubmed-meshheading:9593635-Blotting, Northern,
pubmed-meshheading:9593635-Blotting, Western,
pubmed-meshheading:9593635-Cells, Cultured,
pubmed-meshheading:9593635-Humans,
pubmed-meshheading:9593635-Lysine,
pubmed-meshheading:9593635-Pigment Epithelium of Eye,
pubmed-meshheading:9593635-Platelet-Derived Growth Factor
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pubmed:year |
1998
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pubmed:articleTitle |
The advanced glycation endproduct pentosidine induces the expression of PDGF-B in human retinal pigment epithelial cells.
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pubmed:affiliation |
Department of Ophthalmology, University of California, Davis, CA 95616-8794, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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