Linked Life Data

9588211

Source:http://linkedlifedata.com/resource/pubmed/id/9588211

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1998-6-5
pubmed:abstractText
Endothelin-1 (ET-1) is a peptide hormone with potent vasoconstrictor properties that is synthesized and secreted predominantly by vascular endothelial cells. Its production is regulated by numerous stimuli including ischemia and hypoxia, and the enhanced levels that occur during myocardial ischemia may contribute to the progression of heart failure. We previously reported that ET-1 expression was induced by both hypoxia and transition metals in endothelial cells (ECs). Here we define an element in the proximal promoter of the ET-1 gene that is responsible for this induction. By using deletions and site directed mutagenesis of the human ET-1 promoter, in combination with electrophoretic gel mobility shifts and transient expression assays in human ECs, we identified an active hypoxia-inducible factor 1 (HIF-1) binding site starting at position -118 upstream of the transcription start site on the non-coding DNA strand. Mutation of this site eliminated induction by hypoxia without affecting basal (aerobic) expression, and the mutated sequence did not display hypoxia-specific binding of HIF-1.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
245
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
894-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9588211-Binding Sites, pubmed-meshheading:9588211-Cell Hypoxia, pubmed-meshheading:9588211-DNA-Binding Proteins, pubmed-meshheading:9588211-Endothelin-1, pubmed-meshheading:9588211-Endothelium, Vascular, pubmed-meshheading:9588211-Gene Expression Regulation, pubmed-meshheading:9588211-Helix-Loop-Helix Motifs, pubmed-meshheading:9588211-Humans, pubmed-meshheading:9588211-Hypoxia-Inducible Factor 1, pubmed-meshheading:9588211-Hypoxia-Inducible Factor 1, alpha Subunit, pubmed-meshheading:9588211-Mutagenesis, Site-Directed, pubmed-meshheading:9588211-Nuclear Proteins, pubmed-meshheading:9588211-Oligonucleotides, Antisense, pubmed-meshheading:9588211-Oxygen, pubmed-meshheading:9588211-Promoter Regions, Genetic, pubmed-meshheading:9588211-Sequence Homology, Nucleic Acid, pubmed-meshheading:9588211-Transcription Factors
pubmed:year
1998
pubmed:articleTitle
Hypoxia regulates expression of the endothelin-1 gene through a proximal hypoxia-inducible factor-1 binding site on the antisense strand.
pubmed:affiliation
Department of Molecular and Cellular Pharmacology, University of Miami Medical Center, Florida 33136, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't