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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1998-7-6
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pubmed:abstractText |
Nitric oxide (NO) generation from a spin trap, N-tert-butyl-alpha-phenylnitrone (PBN) under various oxidative conditions was examined. The absorbance of PBN at 295 nm decreased with time of UV-irradiation, showing that PBN was decomposed by UV irradiation. The hydroxyl radical formed from a Fenton reagent also decomposed PBN, but there was little effect by a peroxyl radical and a superoxide. Nitrite, an oxidative product of NO, in PBN solution was determined using a NOx analyzer based on Griess reaction. UV-irradiation and the hydroxyl radical also formed nitrite. Direct detection of NO from the sample on reaction with hydroxyl radical was successful using a GC/MS/SIM on the UV-irradiated sample. NO generated in PBN solutions activated guanylate cyclase. From these results, PBN is viewed as a new kind of medicine which acts as an antioxidant and as an NO donor in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0918-6158
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
401-4
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9586581-Animals,
pubmed-meshheading:9586581-Cyclic GMP,
pubmed-meshheading:9586581-Cyclic N-Oxides,
pubmed-meshheading:9586581-Enzyme Inhibitors,
pubmed-meshheading:9586581-Free Radical Scavengers,
pubmed-meshheading:9586581-Free Radicals,
pubmed-meshheading:9586581-Guanylate Cyclase,
pubmed-meshheading:9586581-Hydroxyl Radical,
pubmed-meshheading:9586581-Liver,
pubmed-meshheading:9586581-Male,
pubmed-meshheading:9586581-Mice,
pubmed-meshheading:9586581-Mice, Inbred C57BL,
pubmed-meshheading:9586581-Nitric Oxide,
pubmed-meshheading:9586581-Nitrogen Oxides,
pubmed-meshheading:9586581-Oxidation-Reduction,
pubmed-meshheading:9586581-Spectrophotometry, Ultraviolet,
pubmed-meshheading:9586581-Spin Trapping
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pubmed:year |
1998
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pubmed:articleTitle |
Release of nitric oxide from a spin trap, N-tert-butyl-alpha-phenylnitrone, under various oxidative conditions.
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pubmed:affiliation |
Gerontology Research Center, National Institute on Aging, NIH, Baltimore, MD 21224, USA.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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