Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-5-19
pubmed:abstractText
Without culture, differentiation of bacille Calmette-Guérin-induced lymphadenitis (BCG-LA) from tuberculosis (TB) is sometimes difficult by histology, but is important because of different treatment schemes. The purpose of this study was to investigate the feasibility of differentiating BCG-LA from TB in lymph nodes (LNs) by molecular analyses of two recently identified genes, pncA and oxyR. In both genes, a single tuberculosis difference exists between Mycobacterium bovis and M. tuberculosis. M tuberculosis complex (MTC) DNA was first detected in nine of ten formalin-fixed, paraffin-embedded LNs from patients aged under 20 years with suspected mycobacterial infections, using polymerase chain reaction (PCR) for IS6110, an insertion sequence specific for MTC species. PCR, together with direct DNA sequencing and PCR-restriction fragment length polymorphism (RFLP) assay, was then performed to identify polymorphic nucleotide in pncA and oxyR, respectively. For comparison, 37 adult cases of tuberculous lymphadenitis were also analysed by PCR-single strand conformation polymorphism (SSCP) assay for pncA and by PCR-RFLP for oxyR. The results revealed that five of the nine IS6110-positive child cases had a G residue at nucleotide 169 in pncA, and also had a three-band pattern after digesting the amplified oxyR segment with AluI, suggesting BCG-LA. The remaining four child cases, as well as all adult cases with detectable IS6110, showed no motility shift in pncA PCR-SSCP and had the same one-band pattern as M. tuberculosis in oxyR PCR-RFLP, suggesting TB lymphadenitis. The data from molecular analyses showed a good correlation with the vaccination history and clinicopathological findings, except for one case. This study indicates that molecular assay of either oxyR or pncA could be a rapid and useful tool to distinguish BCG-LA from TB.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-3417
pubmed:author
pubmed:issnType
Print
pubmed:volume
184
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
96-102
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9582534-Adolescent, pubmed-meshheading:9582534-BCG Vaccine, pubmed-meshheading:9582534-Child, pubmed-meshheading:9582534-Child, Preschool, pubmed-meshheading:9582534-DNA-Binding Proteins, pubmed-meshheading:9582534-Diagnosis, Differential, pubmed-meshheading:9582534-Genes, Bacterial, pubmed-meshheading:9582534-Humans, pubmed-meshheading:9582534-Infant, pubmed-meshheading:9582534-Lymph Nodes, pubmed-meshheading:9582534-Lymphadenitis, pubmed-meshheading:9582534-Mutation, pubmed-meshheading:9582534-Mycobacterium bovis, pubmed-meshheading:9582534-Mycobacterium tuberculosis, pubmed-meshheading:9582534-Polymerase Chain Reaction, pubmed-meshheading:9582534-Polymorphism, Restriction Fragment Length, pubmed-meshheading:9582534-Polymorphism, Single-Stranded Conformational, pubmed-meshheading:9582534-Repressor Proteins, pubmed-meshheading:9582534-Transcription Factors, pubmed-meshheading:9582534-Tuberculosis, Lymph Node
pubmed:year
1998
pubmed:articleTitle
Differentiation of BCG-induced lymphadenitis from tuberculosis in lymph node biopsy specimens by molecular analyses of pncA and oxyR.
pubmed:affiliation
Department of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't