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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1998-7-1
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pubmed:abstractText |
The elaborate and energy-intensive spliceosome assembly pathway belies the seemingly simple chemistry of pre-mRNA splicing. Prp38p was previously identified as a protein required in vivo and in vitro for the first pre-mRNA cleavage reaction catalyzed by the spliceosome. Here we show that Prp38p is a unique component of the U4/U6.U5 tri-small nuclear ribonucleoprotein (snRNP) particle and is necessary for an essential step late in spliceosome maturation. Without Prp38p activity spliceosomes form, but arrest in a catalytically impaired state. Functional spliceosomes shed U4 snRNA before 5' splice-site cleavage. In contrast, Prp38p-defective spliceosomes retain U4 snRNA bound to its U6 snRNA base-pairing partner. Prp38p is the first tri-snRNP-specific protein shown to be dispensable for assembly, but required for conformational changes which lead to catalytic activation of the spliceosome.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-1396567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-1464325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-1508195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-15336150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-1830649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-1831175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-1833635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-1882420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-1996139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-2010088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-2145195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-2147224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-2304461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-2478298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-2528687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-2953438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-2962902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-2997224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-3030557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-3073106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-3286372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-3315850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-3537805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-3638792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-6092912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-6308621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-7489518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-7493316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-7520570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-7534458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-7585243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-7585252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-7882985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-8112301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-8146658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-8196608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-8233807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-8248176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-8430095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-8430102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-8479905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-8649518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-8670905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-8682289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-8722763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-8752092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-8811184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-8943336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-8969184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-9042946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-9085842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-9233817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-9342317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9582287-9418882
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0261-4189
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2938-46
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1998
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pubmed:articleTitle |
Progression through the spliceosome cycle requires Prp38p function for U4/U6 snRNA dissociation.
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pubmed:affiliation |
T.H. Morgan School of Biological Sciences, University of Kentucky, Lexington, KY 40506-0225, USA.
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pubmed:publicationType |
Journal Article
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