Linked Life Data

9581680

Source:http://linkedlifedata.com/resource/pubmed/id/9581680

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1998-5-19
pubmed:abstractText
Effects of antioxidants, resveratrol, quercetin, and N-acetylcysteine (NAC) on the functions of cultured rat hepatic stellate cells and Kupffer cells were studied. These compounds dose-dependently suppressed serum-dependent proliferation of stellate cells as determined by [3H]thymidine and 5-bromo-2'-deoxyuridine uptake. Expression of smooth muscle alpha-actin was suppressed by a high dose of resveratrol and quercetin. These phenolic compounds also suppressed inositol phosphate metabolism, tyrosine phosphorylation, and mitogen-activated protein (MAP) kinase activation in platelet-derived growth factor/BB-stimulated stellate cells. Moreover, the phenolic compounds selectively reduced the level of cell cycle protein cyclin D1 in stellate cells. Thus, resveratrol and quercetin might inhibit stellate cell activation by perturbing signal transduction pathway and cell cycle protein expression, whereas mechanism of potent antiproliferative effect of NAC remains to be elucidated. On the other hand, kinetic analysis showed that production of nitric oxide (NO) and tumor necrosis factor alpha (TNF-alpha) by lipopolysaccharide-stimulated Kupffer cells was strongly inhibited by resveratrol and quercetin but not by NAC. Although expression of messenger RNAs for inducible NO synthase and TNF-alpha was not affected by the phenolic compounds, cellular levels of inducible NO synthase and TNF-alpha secretion were suppressed significantly, indicating the posttranscriptional process of generating these proteins might be affected predominantly by these phenolic compounds. Thus, NAC and these phenolic compounds may have therapeutic potential against liver injury by regulating functions of hepatic stellate cells and Kupffer cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0270-9139
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1265-74
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:9581680-Actins, pubmed-meshheading:9581680-Animals, pubmed-meshheading:9581680-Antioxidants, pubmed-meshheading:9581680-Calcium-Calmodulin-Dependent Protein Kinases, pubmed-meshheading:9581680-Cell Cycle, pubmed-meshheading:9581680-Cell Division, pubmed-meshheading:9581680-Gene Expression Regulation, pubmed-meshheading:9581680-Inositol Phosphates, pubmed-meshheading:9581680-Kupffer Cells, pubmed-meshheading:9581680-Liver, pubmed-meshheading:9581680-Male, pubmed-meshheading:9581680-Nitric Oxide, pubmed-meshheading:9581680-Phosphotyrosine, pubmed-meshheading:9581680-Quercetin, pubmed-meshheading:9581680-RNA, Messenger, pubmed-meshheading:9581680-Rats, pubmed-meshheading:9581680-Rats, Wistar, pubmed-meshheading:9581680-Stilbenes, pubmed-meshheading:9581680-Tumor Necrosis Factor-alpha
pubmed:year
1998
pubmed:articleTitle
Effect of antioxidants, resveratrol, quercetin, and N-acetylcysteine, on the functions of cultured rat hepatic stellate cells and Kupffer cells.
pubmed:affiliation
Department of Internal Medicine, Osaka City University Medical School, Abeno, Osaka, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't