Linked Life Data

9581624

Source:http://linkedlifedata.com/resource/pubmed/id/9581624

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-6-17
pubmed:abstractText
Silver-stained nucleolar organizer regions in interphase cells have been widely studied as a cell kinetic parameter in different tissues. Many different approaches have been used for this evaluation, often leading to conflicting results. The aim of our study was to analyze several commonly used AgNOR parameters in bone marrow smears of 32 newly diagnosed cases of acute leukemia: number of clusters and dots, percentage of cells with one cluster (cells in a long G1 phase), total and percentage of silver-stained area per nucleus, mean size of AgNORs, and the Rüschoff index. These parameters were compared with the in vitro incorporation of bromodeoxyuridine (BrdU). A significant correlation was found between the BrdU index and the mean number of clusters (r = 0.60), dots (r = 0.60), percentage of cells with one cluster (r = -0.63), as well as with the mean size of AgNORs (r = -0.63) and the Rüschoff index (r = 0.72). In a factor analysis, total AgNOR area, its percentage of nuclear area, the mean AgNOR size, and the nuclear area formed one factor (factor of area parameters), and clusters, dots, the percentage of cells with one cluster, and BrdU index made another factor (factor of proliferation parameters). The Rüschoff index participated equally in both factors. Based on these data, we postulate that the number of clusters and dots are related to the percentage of cells in S-phase and the AgNOR area may be related to the cell duplication time, as has been reported in the literature. Mean size of AgNORs is smaller in highly proliferating cells. Probably the Rüschoff index is related to the percentage of cells in S-phase as well as to the cell duplication time, and seems to be the best AgNOR parameter for the global evaluation of cell kinetics in acute leukemias.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0196-4763
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
51-6
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Relationship between morphometric analysis of nucleolar organizer regions and cell proliferation in acute leukemias.
pubmed:affiliation
Department of Internal Medicine, School of Medicine, State University of Campinas, São Paulo, Brazil. ilmetze@obelix.unicamp.br
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't