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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1998-5-20
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pubmed:abstractText |
The effect of cycloheximide (CHX) on the mRNA expression of the cytokine-inducible, calcium-independent nitric oxide synthase (iNOS) was investigated in fetal hepatocytes stimulated with lipopolysaccharide (LPS) or pro-inflammatory cytokines. In the presence of CHX the LPS-dependent iNOS mRNA levels were reduced, whereas the response to pro-inflammatory cytokines was enhanced. Because iNOS transcription is highly dependent on the activation of nuclear factor kappaB (NF-kappaB), this factor was evaluated by electrophoretic mobility shift assays, and a close correlation between NF-kappaB activity and iNOS mRNA levels was observed. CHX itself potentiated the degradation of the IkappaB alpha and IkappaB beta inhibitory subunits (IkappaB is inhibitory kappaB) of the NF-kappaB complex, and therefore the loss of LPS-dependent iNOS mRNA expression cannot be attributed to a blockage in the activation of NF-kappaB. These results suggest the existence of a CHX-sensitive pathway in the expression of iNOS mediated by LPS, a mechanism that is not involved in the response to pro-inflammatory cytokines.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-1373522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-1378626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-1381691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-1712077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-1852778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-2000146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-2107492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-2539097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-2771659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-7508926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-7684434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-7741027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-7775482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-7814425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-7867065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-8011280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-8122310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-8561789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-8595383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-8603706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-8645258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-8687469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-8697149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9581561-8939960
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/I kappa B beta protein,
http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Inflammation Mediators,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappaB inhibitor alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Nos2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Synthesis Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
327 ( Pt 3)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
819-23
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9581561-Animals,
pubmed-meshheading:9581561-Cycloheximide,
pubmed-meshheading:9581561-Cytokines,
pubmed-meshheading:9581561-DNA-Binding Proteins,
pubmed-meshheading:9581561-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:9581561-I-kappa B Proteins,
pubmed-meshheading:9581561-Inflammation Mediators,
pubmed-meshheading:9581561-Interleukin-1,
pubmed-meshheading:9581561-Lipopolysaccharides,
pubmed-meshheading:9581561-Liver,
pubmed-meshheading:9581561-NF-kappa B,
pubmed-meshheading:9581561-Nitric Oxide Synthase,
pubmed-meshheading:9581561-Nitric Oxide Synthase Type II,
pubmed-meshheading:9581561-Protein Synthesis Inhibitors,
pubmed-meshheading:9581561-RNA, Messenger,
pubmed-meshheading:9581561-Rats,
pubmed-meshheading:9581561-Rats, Wistar,
pubmed-meshheading:9581561-Transcription, Genetic,
pubmed-meshheading:9581561-Transcription Factor AP-1,
pubmed-meshheading:9581561-Tumor Necrosis Factor-alpha
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pubmed:year |
1997
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pubmed:articleTitle |
Differential regulation of nitric oxide synthase mRNA expression by lipopolysaccharide and pro-inflammatory cytokines in fetal hepatocytes treated with cycloheximide.
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pubmed:affiliation |
Instituto de Bioquímica (CSIC-UCM), Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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