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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1998-6-23
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pubmed:databankReference |
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pubmed:abstractText |
We describe the characterization of a gene, Pad-1, from Neurospora crassa which displays sequence characteristics of the RS class of hnRNA-binding proteins (hnRNP) and mRNA splicing factors. This is the first report of the isolation of a putative hnRNP gene from N. crassa. PAD-1 showed 30% identity and 57% similarity to a protein, HCC1, which was isolated using autoantibodies from patients suffering from hepatocellular carcinoma. Both HCC1 and PAD-1 show amino acid sequence similarities to the human splicing factor, U2AF65. Mutations induced in Pad-1 by repeat-induced point (RIP) mutation show dominant effects on ascus and ascospore formation, a novel phenotypic class of RIP mutants. A mutant isolated from the Pad-1 RIP cross displayed a severe vegetative growth defect and dominant effects on ascus development, indicating that Pad-1 is essential for both asexual and sexual development.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HCC1 autoantigen,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Fungal,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins, Small Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/splicing factor U2AF
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1087-1845
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1998 Academic Press.
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pubmed:issnType |
Print
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
169-80
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:9578630-Amino Acid Sequence,
pubmed-meshheading:9578630-Base Sequence,
pubmed-meshheading:9578630-Cloning, Molecular,
pubmed-meshheading:9578630-Fungal Proteins,
pubmed-meshheading:9578630-Genes, Dominant,
pubmed-meshheading:9578630-Genes, Fungal,
pubmed-meshheading:9578630-Humans,
pubmed-meshheading:9578630-Molecular Sequence Data,
pubmed-meshheading:9578630-Mutagenesis,
pubmed-meshheading:9578630-Neurospora crassa,
pubmed-meshheading:9578630-Nuclear Proteins,
pubmed-meshheading:9578630-Point Mutation,
pubmed-meshheading:9578630-RNA, Fungal,
pubmed-meshheading:9578630-RNA, Messenger,
pubmed-meshheading:9578630-RNA, Nuclear,
pubmed-meshheading:9578630-RNA Splicing,
pubmed-meshheading:9578630-RNA-Binding Proteins,
pubmed-meshheading:9578630-Ribonucleoproteins,
pubmed-meshheading:9578630-Ribonucleoproteins, Small Nuclear,
pubmed-meshheading:9578630-Sequence Alignment,
pubmed-meshheading:9578630-Spores, Fungal
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pubmed:year |
1998
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pubmed:articleTitle |
Repeat-induced point mutations in Pad-1, a putative RNA splicing factor from Neurospora crassa, confer dominant lethal effects on ascus development.
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pubmed:affiliation |
Botany Department and Biotechnology Laboratory, University of British Columbia, Vancouver, Canada.
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pubmed:publicationType |
Journal Article
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