Linked Life Data

9578137

Source:http://linkedlifedata.com/resource/pubmed/id/9578137

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-7-28
pubmed:abstractText
Age differences in the expression of cerebellar metabotropic glutamate receptor 1 (mGluR1) and inositol 1,4,5-trisphosphate receptor (IP3R) were investigated using male C57BL/6NNIA mice 5, 15 and 24 months of age. In situ hybridization for mGluR1 mRNA in the granule cell layer indicated significantly higher mRNA levels in the 24-month-old group as compared to the 5- and 15-month-old groups. However, mRNA levels of individual Purkinje neurons did not show age differences. Western blot analysis using antibody against the predominant isoform, mGluR1a, showed a decline in protein levels in the 24-month-old animals. In situ hybridization for IP3R type 1 mRNA in Purkinje neurons showed a slight but not significant decline in the 24-month-old group. Further assay of [3H]IP3 binding with cerebellar membranes showed significant reduction in Bmax values in the 15- and 24-month-old groups as compared to the 5-month-old group but Kd values were not changed. The decrease in mGluR1a receptor protein together with reduction in IP3R binding sites may play an important role in the decline in cerebellar functions with increasing age.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0304-3940
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
244
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
29-32
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Age differences in the expression of metabotropic glutamate receptor 1 and inositol 1,4,5-trisphosphate receptor in mouse cerebellum.
pubmed:affiliation
Biochemistry Department, University of Missouri, Columbia 65212, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't