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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
Pt 11
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pubmed:dateCreated |
1998-7-17
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pubmed:abstractText |
The mechanism of action of synthetic CCRF-DP, the corticotropin-releasing factor (CRF)-related diuretic peptide of the salt marsh mosquito Culex salinarius, was investigated in isolated Malpighian tubules of the yellow fever mosquito Aedes aegypti. A low concentration of CCRF-DP (10(-9)mol l-1) caused a small but insignificant increase in transepithelial secretion of NaCl and fluid, but significantly reduced transepithelial voltage and resistance without a change in short-circuit current, pointing to the stimulation of passive Cl- transport through the paracellular pathway as the principal mechanism of a mild diuresis. Significant changes in voltage and resistance but not in short-circuit current were duplicated by the ionophore A23187 (0.4 micromol l-1), suggesting Ca2+ as a second messenger at 10(-9)mol l-1 CCRF-DP. A high concentration of CCRF-DP (10(-7)mol l-1) significantly increased transepithelial secretion of NaCl and fluid and significantly increased short-circuit current, pointing to the stimulation of active Na+ transport through the transcellular pathway as the mechanism of a strong diuresis. This effect was mimicked by dibutyryl-cAMP, suggesting cAMP as a second messenger at 10(-7)mol l-1 CCRF-DP. Dibutyryl-cGMP had no effects. These results suggest dose-dependent, receptor-mediated effects of CCRF-DP that target discrete transport pathways via discrete second messengers: low concentrations of CCRF-DP cause a mild diuresis, apparently via Ca2+-mediated effects on paracellular Cl- transport, and high concentrations cause a strong diuresis via cAMP-mediated effects on active transcellular Na+ transport in addition to the effects on the paracellular pathway.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bucladesine,
http://linkedlifedata.com/resource/pubmed/chemical/Calcimycin,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Corticotropin-Releasing Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Insect Hormones,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Chloride
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-0949
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
201
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1753-62
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9576886-Aedes,
pubmed-meshheading:9576886-Animals,
pubmed-meshheading:9576886-Bucladesine,
pubmed-meshheading:9576886-Calcimycin,
pubmed-meshheading:9576886-Calcium,
pubmed-meshheading:9576886-Chlorides,
pubmed-meshheading:9576886-Corticotropin-Releasing Hormone,
pubmed-meshheading:9576886-Diuresis,
pubmed-meshheading:9576886-Electric Conductivity,
pubmed-meshheading:9576886-Electrophysiology,
pubmed-meshheading:9576886-Epithelium,
pubmed-meshheading:9576886-Insect Hormones,
pubmed-meshheading:9576886-Malpighian Tubules,
pubmed-meshheading:9576886-Second Messenger Systems,
pubmed-meshheading:9576886-Sodium Chloride
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pubmed:year |
1998
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pubmed:articleTitle |
The concentration-dependence of CRF-like diuretic peptide: mechanisms of action.
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pubmed:affiliation |
Department of Zoology, Washington State University, Pullman, WA 99164, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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