Linked Life Data

9576772

Source:http://linkedlifedata.com/resource/pubmed/id/9576772

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-8-31
pubmed:databankReference
pubmed:abstractText
Previously we reported that oxalate oxidase activity increases in extracts of barley (Hordeum vulgare) leaves in response to the powdery mildew fungus (Blumeria [syn. Erysiphe] graminis f.sp. hordei) and proposed this as a source of H2O2 during plant-pathogen interactions. In this paper we show that the N terminus of the major pathogen-response oxalate oxidase has a high degree of sequence identity to previously characterized germin-like oxalate oxidases. Two cDNAs were isolated, pHvOxOa, which represents this major enzyme, and pHvOxOb', representing a closely related enzyme. Our data suggest the presence of only two oxalate oxidase genes in the barley genome, i.e. a gene encoding HvOxOa, which possibly exists in several copies, and a single-copy gene encoding HvOxOb. The use of 3' end gene-specific probes has allowed us to demonstrate that the HvOxOa transcript accumulates to 6 times the level of the HvOxOb transcript in response to the powdery mildew fungus. The transcripts were detected in both compatible and incompatible interactions with a similar accumulation pattern. The oxalate oxidase is found exclusively in the leaf mesophyll, where it is cell wall located. A model for a signal transduction pathway in which oxalate oxidase plays a central role is proposed for the regulation of the hypersensitive response.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9576772-16668394, http://linkedlifedata.com/resource/pubmed/commentcorrection/9576772-1668186, http://linkedlifedata.com/resource/pubmed/commentcorrection/9576772-6546423, http://linkedlifedata.com/resource/pubmed/commentcorrection/9576772-8012045, http://linkedlifedata.com/resource/pubmed/commentcorrection/9576772-8044704, http://linkedlifedata.com/resource/pubmed/commentcorrection/9576772-8143935, http://linkedlifedata.com/resource/pubmed/commentcorrection/9576772-8159797, http://linkedlifedata.com/resource/pubmed/commentcorrection/9576772-8400378, http://linkedlifedata.com/resource/pubmed/commentcorrection/9576772-8509360, http://linkedlifedata.com/resource/pubmed/commentcorrection/9576772-8723347, http://linkedlifedata.com/resource/pubmed/commentcorrection/9576772-8736791, http://linkedlifedata.com/resource/pubmed/commentcorrection/9576772-9049263, http://linkedlifedata.com/resource/pubmed/commentcorrection/9576772-9484466
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
117
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
33-41
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Molecular characterization of the oxalate oxidase involved in the response of barley to the powdery mildew fungus.
pubmed:affiliation
Plant Pathology Section, Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Copenhagen, Denmark.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't