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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1998-5-19
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pubmed:abstractText |
Endothelial cells in normal blood vessels might prevent the unscheduled proliferation of smooth muscle cells (SMCs) by the expression of cell migration and growth inhibitors. NO, a potent vasodilator, generated by endothelium-specific constitutive NO synthase (ecNOS) might be such an inhibitor. To test this hypothesis, we overexpressed human ecNOS in syngeneic rat arterial SMCs using retrovirus-mediated gene transfer. Compared with SMCs transduced with vector alone (LXSN SMCs), DNA synthesis and cell proliferation were inhibited in the ecNOS-expressing SMCs (LCNSN SMCs). Basal and stimulated (by the calcium ionophore A23187) secretion of NO and intracellular cGMP were increased in LCNSN SMCs. Nomega-Nitro-L-arginine (L-NA), an inhibitor of NO synthesis, enhanced the proliferation of LCNSN SMCs but had no effect on LXSN SMCs. LCNSN SMCs seeded onto the luminal surface of balloon-injured rat carotid arteries inhibited neointimal formation by 37% and induced marked dilatation (3-fold increase in vessel diameter) at 2 weeks compared with LXSN SMC-seeded arteries. Orally administered L-NA blocked these changes. Phosphorylation of vasodilator-stimulated phosphoprotein, which is regulated in part by NO, was elevated in LCNSN SMCs and in LCNSN SMC-seeded arteries. This study demonstrates that NO generation by ecNOS inhibits SMC proliferation in vitro and modulates vascular tone locally in vivo.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcimycin,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic GMP,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Microfilament Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NOS3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type III,
http://linkedlifedata.com/resource/pubmed/chemical/Nitroarginine,
http://linkedlifedata.com/resource/pubmed/chemical/Nos3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/vasodilator-stimulated...
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0009-7330
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
862-70
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9576106-Angioplasty, Balloon,
pubmed-meshheading:9576106-Animals,
pubmed-meshheading:9576106-Aorta,
pubmed-meshheading:9576106-Calcimycin,
pubmed-meshheading:9576106-Carotid Arteries,
pubmed-meshheading:9576106-Carotid Artery Injuries,
pubmed-meshheading:9576106-Cell Adhesion Molecules,
pubmed-meshheading:9576106-Cell Division,
pubmed-meshheading:9576106-Cells, Cultured,
pubmed-meshheading:9576106-Cyclic GMP,
pubmed-meshheading:9576106-Endothelium, Vascular,
pubmed-meshheading:9576106-Gene Transfer Techniques,
pubmed-meshheading:9576106-Humans,
pubmed-meshheading:9576106-Immunohistochemistry,
pubmed-meshheading:9576106-Isoenzymes,
pubmed-meshheading:9576106-Kinetics,
pubmed-meshheading:9576106-Male,
pubmed-meshheading:9576106-Microfilament Proteins,
pubmed-meshheading:9576106-Muscle, Smooth, Vascular,
pubmed-meshheading:9576106-Nitric Oxide Synthase,
pubmed-meshheading:9576106-Nitric Oxide Synthase Type III,
pubmed-meshheading:9576106-Nitroarginine,
pubmed-meshheading:9576106-Phosphoproteins,
pubmed-meshheading:9576106-Rats,
pubmed-meshheading:9576106-Rats, Inbred F344,
pubmed-meshheading:9576106-Recombinant Proteins,
pubmed-meshheading:9576106-Retroviridae,
pubmed-meshheading:9576106-Transfection
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pubmed:year |
1998
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pubmed:articleTitle |
Overexpression of human endothelial nitric oxide synthase in rat vascular smooth muscle cells and in balloon-injured carotid artery.
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pubmed:affiliation |
Department of Surgery, University of Washington, Seattle 98195, USA. lihua@u.washington.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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