Linked Life Data

9573364

Source:http://linkedlifedata.com/resource/pubmed/id/9573364

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-6-19
pubmed:abstractText
There is evidence suggesting reciprocal trophic interactions between photoreceptors and the retinal pigmented epithelium (RPE), but the factors involved have not been identified. In this study, we investigated the hypothesis that one or more known neurotrophic factors act upon the RPE. Cultured human and freshly isolated bovine RPE cells demonstrated saturable specific binding for [125I]labeled BDNF, NT-4/5 and NT-3 with little specific binding for CNTF and none for NGF. Cross-competition experiments showed that BDNF is the preferred ligand and cross-linking of [125I]BDNF resulted in a doublet at 160 kd that was increased in RPE cells incubated in all-trans retinoic acid. There was basal phosphorylation of a 145 kd protein recognized by an anti-trk antibody that was increased in RPE cells pulsed with BDNF. RT-PCR with primers spanning the transmembrane domain demonstrated that RPE cells express trkB mRNA lacking a region homologous to exon 9 of chicken trkB, a splice variant that has been demonstrated to preferentially interact with BDNF. Northern blots demonstrated that cultured RPE cells also express mRNA for BDNF. BDNF did not stimulate proliferation or increase survival of RPE cells in serum-free medium, but promoted a differentiated morphology and increased the expression of cellular retinaldehyde binding protein, a marker of the differentiated state in RPE cells. An RPE cell line that spontaneously shows differentiated features showed a high level of BDNF mRNA. These data demonstrate that RPE cells express a short splice variant of trkB whose activation correlates with expression of differentiated characteristics and the cells themselves are capable of producing a ligand for the receptors. Signaling through trkB could play a role in differentiation of RPE cells during development and maintenance of the differentiated state in adult RPE.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-8993
pubmed:author
pubmed:copyrightInfo
Copyright 1998 Elsevier Science B.V.
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
789
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
201-12
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9573364-Animals, pubmed-meshheading:9573364-Base Sequence, pubmed-meshheading:9573364-Brain-Derived Neurotrophic Factor, pubmed-meshheading:9573364-Cell Differentiation, pubmed-meshheading:9573364-Cell Survival, pubmed-meshheading:9573364-Cells, Cultured, pubmed-meshheading:9573364-Culture Media, Serum-Free, pubmed-meshheading:9573364-DNA, Recombinant, pubmed-meshheading:9573364-Gene Expression, pubmed-meshheading:9573364-Genetic Variation, pubmed-meshheading:9573364-Humans, pubmed-meshheading:9573364-Mice, pubmed-meshheading:9573364-Molecular Sequence Data, pubmed-meshheading:9573364-Nerve Growth Factors, pubmed-meshheading:9573364-Phenotype, pubmed-meshheading:9573364-Phosphorylation, pubmed-meshheading:9573364-Pigment Epithelium of Eye, pubmed-meshheading:9573364-Receptor, Ciliary Neurotrophic Factor, pubmed-meshheading:9573364-Receptor Protein-Tyrosine Kinases, pubmed-meshheading:9573364-Receptors, Nerve Growth Factor, pubmed-meshheading:9573364-Tyrosine
pubmed:year
1998
pubmed:articleTitle
A splice variant of trkB and brain-derived neurotrophic factor are co-expressed in retinal pigmented epithelial cells and promote differentiated characteristics.
pubmed:affiliation
Department of Ophthalmology, Johns Hopkins University School of Medicine, 600 N. Wolfe Street, Baltimore, MD 21287-9277, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't