Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1998-5-12
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ002446, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ002447, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ002448, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ002449, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ002450, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ002451, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ002452, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ002453, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ002454, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ002455, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ002456, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ002457, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ002458, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ002459, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ002460
pubmed:abstractText
Complete DNA-A sequences of nine Pakistani geminivirus isolates from leaf curl-affected cotton (CLCuV-PK) or from okra, and the partial sequences of several additional isolates were determined. Sequences of isolates from cotton were of four types. Isolates from leaf curl-affected okra had virtually the same sequences as those from cotton. Isolates from yellow vein mosaic-affected okra were of two types (OYVMV types 201 and 301), both distinct from but closely related to the virus isolates from cotton. Of these six types, two types of CLCuV-PK are the most closely related but another (CLCuV-PK type 72b) is the most distinct. Of the encoded proteins, coat protein (CP) is the most strongly conserved (92-100% amino acid sequence identity), and AC4 protein the most variable (41-87%). The 5' and 3' halves of the intergenic region of some isolates had different affinities and occurred in seven combinations, suggesting that recombination had occurred and that the origin of replication was a favoured recombination site. Similarly, the first 1520 nt of CLCuV-PK type 804a DNA resembled those of OYVMV type 301 DNA but the remaining 1224 nt were very different. The AC1 (Rep) gene and 5' part of the intergenic region of CLCuV-PK type 72b closely resembled those of OYVMV type 301, whereas the rest of the sequence did not. The cotton leaf curl epidemic in Pakistan is caused by several distinct variants, with recombination events involving OYVMV and other unspecified geminiviruses having probably been involved in their evolution.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-1317
pubmed:author
pubmed:issnType
Print
pubmed:volume
79 ( Pt 4)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
915-23
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Four DNA-A variants among Pakistani isolates of cotton leaf curl virus and their affinities to DNA-A of geminivirus isolates from okra.
pubmed:affiliation
Scottish Crop Research Institute, Invergowrie, Dundee, UK.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't