Linked Life Data

9568742

Source:http://linkedlifedata.com/resource/pubmed/id/9568742

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-6-11
pubmed:abstractText
The present study was conducted to determine whether alimentary lipemia alters platelet activity in vivo. Normolipidemic volunteers were given a fatty meal and platelet function was assessed before, and 3 and 6 h after the meal. Platelet aggregability and secretion was determined using whole blood flow cytometry (expression of platelet P-selectin and fibrinogen binding), filtragometry ex vivo (reflecting platelet aggregability in vivo) and by measurements of platelet specific products in plasma (beta-thromboglobulin and platelet factor 4). Plasma triglycerides increased from 0.8 (0.6:1.1; median, 25th and 75th percentiles) to 1.7 (1.0:2.3) mmol/l at 3 h and returned to baseline after 6 h (P < 0.001, one-way ANOVA). Apo B-100 and apo B-48 were both markedly increased 3 h postprandially in the Sf 60-400 fraction (large VLDLs, P < 0.001 for both), whereas the Sf 20-60 (small VLDLs) and Sf 12-20 fractions (IDL) did not change. The platelet function assessments revealed that the percentage of platelets expressing P-selectin increased by 40% (5%; 64%) after 3 h and by 51% (- 7%; 85%) 6 h postprandially in unstimulated samples (P < 0.05 for both). In samples stimulated by ADP in vitro P-selectin expression increased by 45% (6%; 58%) after 3 h and by 30% (12%; 58%) (P<0.01 for both) after 6 h at 0.1 microM. Platelet P-selectin expression was less influenced at higher ADP concentrations. The plasma levels of beta-thromboglobulin (approximately 20 ng/ml) and platelet factor 4 (approximately 0.3 ng/ml) were not affected by the fat load. Flow cytometric analyses of fibrinogen binding and filtragometry measurements also failed to reveal any postprandial alterations. The present finding of enhanced platelet P-selectin expression suggests that platelets are mildly sensitized postprandially. Whether this is of importance for thrombus formation and atherosclerosis needs to be studied further.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9150
pubmed:author
pubmed:issnType
Print
pubmed:volume
137
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
107-13
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9568742-Adult, pubmed-meshheading:9568742-Apolipoproteins B, pubmed-meshheading:9568742-Biological Markers, pubmed-meshheading:9568742-Blood Platelets, pubmed-meshheading:9568742-Cell Membrane, pubmed-meshheading:9568742-Data Interpretation, Statistical, pubmed-meshheading:9568742-Diet, Atherogenic, pubmed-meshheading:9568742-Dietary Fats, pubmed-meshheading:9568742-Humans, pubmed-meshheading:9568742-Hyperlipidemias, pubmed-meshheading:9568742-Hypertriglyceridemia, pubmed-meshheading:9568742-Lipids, pubmed-meshheading:9568742-Lipoproteins, pubmed-meshheading:9568742-Male, pubmed-meshheading:9568742-P-Selectin, pubmed-meshheading:9568742-Platelet Activation, pubmed-meshheading:9568742-Platelet Factor 4, pubmed-meshheading:9568742-Postprandial Period, pubmed-meshheading:9568742-Time Factors, pubmed-meshheading:9568742-Triglycerides, pubmed-meshheading:9568742-beta-Thromboglobulin
pubmed:year
1998
pubmed:articleTitle
Alimentary lipemia enhances the membrane expression of platelet P-selectin without affecting other markers of platelet activation.
pubmed:affiliation
Department of Laboratory Medicine, Karolinska Institute & Hospital, Stockholm, Sweden. Anders.Broijersen@labmed.ki.se
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't