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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1998-6-8
|
| pubmed:abstractText |
The importance of bioassays measuring stimulating and blocking autoantibodies to the TSH-receptor (TSH-R) by their effect on cAMP production in CHO cells transfected with the recombinant TSH-R is increasingly recognized. The standard technique for this bioassay is cumbersome, as it involves purification of serum IgG with polyethylene glycol (PEG) and resuspension in hypotonic buffer. We have therefore established a simpler approach for the detection of stimulating and blocking autoantibodies using JP09 CHO cells and unfractionated human serum. The cAMP concentration was measured by a highly sensitive commercial radioimmuno assay. Thyroid stimulating autoantibodies (TSAb) were present in 107 out of 126 patients with Graves' disease (85%) and in 4 out of 40 patients with Hashimoto's thyroiditis (10%). Specificity was confirmed by the fact that only 1 patient with insulin dependent diabetes mellitus (IDDM) out of 64 patients with different non-thyroid autoimmune disorders (46 with IDDM, 10 with stiff man syndrome and 8 with rheumatoid arthritis) and 2 out of 100 healthy controls (2%) were positive in this assay. In the subgroup of hyperthyroid Graves' disease patients 76 out of 83 (92%) had TSAb and the same number had TSH binding inhibiting immunoglobulin (TBII), as assessed by the commercial TRAK assay. Although both antibody types showed only a weak correlation (r = 0.30), a combination of TSAb and TBII detected 98% of all Graves' patients and 99% of the hyperthyroid subgroup. Thyroid blocking autoantibodies (TBAb) were measured in 4 out of 24 TSAb negative patients with Graves' disease (17%), who were hypothyroid and positive for TBII. A comparison of our bioassay with the standard bioassay using PEG precipitation showed a good correlation (r = 0.76,p < 0.001), demonstrating the feasibility of the simplified assay for the routine detection of TSAb and TBAb in Graves' disease.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Autoantibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulins, Thyroid-Stimulating,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Thyrotropin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/thyrotropin-binding inhibitory...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0018-5043
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
30
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
162-8
|
| pubmed:dateRevised |
2009-2-19
|
| pubmed:meshHeading |
pubmed-meshheading:9566861-Adult,
pubmed-meshheading:9566861-Aged,
pubmed-meshheading:9566861-Aged, 80 and over,
pubmed-meshheading:9566861-Animals,
pubmed-meshheading:9566861-Autoantibodies,
pubmed-meshheading:9566861-Biological Assay,
pubmed-meshheading:9566861-CHO Cells,
pubmed-meshheading:9566861-Cricetinae,
pubmed-meshheading:9566861-Cyclic AMP,
pubmed-meshheading:9566861-Graves Disease,
pubmed-meshheading:9566861-Humans,
pubmed-meshheading:9566861-Immunoglobulins, Thyroid-Stimulating,
pubmed-meshheading:9566861-Middle Aged,
pubmed-meshheading:9566861-ROC Curve,
pubmed-meshheading:9566861-Radioimmunoassay,
pubmed-meshheading:9566861-Receptors, Thyrotropin,
pubmed-meshheading:9566861-Recombinant Proteins,
pubmed-meshheading:9566861-Sensitivity and Specificity,
pubmed-meshheading:9566861-Thyroiditis, Autoimmune,
pubmed-meshheading:9566861-Transfection
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Application of a bioassay with CHO cells for the routine detection of stimulating and blocking autoantibodies to the TSH-receptor.
|
| pubmed:affiliation |
Department of Internal Medicine III, University of Leipzig, Germany.
|
| pubmed:publicationType |
Journal Article
|