Linked Life Data

9566520

Source:http://linkedlifedata.com/resource/pubmed/id/9566520

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-6-23
pubmed:abstractText
During intracellular development of the malarial parasite numerous membranous vesicles appear in the infected erythrocyte cytoplasm between the parasitophorous vacuolar membrane (PVM) and the erythrocyte plasma membrane. In this study we describe the characterization of a monoclonal antibody which recognizes two major parasite-encoded proteins of 50 and 41 kDa. Immunofluorescence and immunoelectron microscopy demonstrated that the monoclonal antibody reacts with cytoplasmic vesicles of Plasmodium falciparum infected erythrocyte referred to as Maurer's clefts. The antigens recognized by the monoclonal antibody were expressed very early during the erythrocytic life cycle of the parasite, and remained tightly associated within membrane vesicles even after merozoites are released from infected erythrocytes. The antigens were partially soluble in non-ionic detergents, and were released from the membrane by alkali treatment, indicating that the proteins recognized by the monoclonal antibody are peripheral membrane proteins. It is proposed that the 50 and 41 kDa antigens might be part of an underlying membrane skeletal network that provides structural support to vesicles and tubules present in the infected erythrocyte cytoplasm.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0166-6851
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
273-80
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Identification of peripheral membrane proteins associated with the tubo-vesicular network of Plasmodium falciparum infected erythrocytes.
pubmed:affiliation
Laboratorio de Biología Celular, Instituto Nacional de Salud, Bogotá, Colombia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't