Linked Life Data

9565698

Source:http://linkedlifedata.com/resource/pubmed/id/9565698

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-5-18
pubmed:abstractText
H2O2 is a key substance in the oxidative stress. To evaluate the antioxidant activity of intact human umbilical vein endothelial cells (HUVEC), we measured the H2O2 removal rate by the cell in monolayer culture at various H2O2 concentrations (1-300 microM). It was shown that the removal reaction can be divided into two kinetically different reactions: reaction 1 apparently following the Michaelis-Menten kinetics and reaction 2 following the first-order kinetics. Reaction 1, which was diminished by treatment with diethyl maleate, could be attributed to GPx. Reaction 2, which was inhibited by aminotriazole, was principally attributable to catalase, though non-enzymatic reactions may contribute to it partially. Furthermore, we have constructed a mathematical model for the H2O2 elimination including the pentose phosphate pathway enzymes, GSSG reductase and GSH peroxidase. On the basis of the known kinetics and observed activities of the enzymes, the model could reproduce well the observed concentration dependence of the H2O2 removal rate. It was suggested from the simulation study that GSSG reductase is more important than G6PD in determining the rate of the NADPH-dependent H2O2 elimination.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-3002
pubmed:author
pubmed:copyrightInfo
Copyright 1998 Elsevier Science B.V.
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
1380
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
275-88
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Kinetics of hydrogen peroxide elimination by human umbilical vein endothelial cells in culture.
pubmed:affiliation
Center for Humanity and Sciences, Ibaraki Prefectural University of Health Sciences, Ami, Ibaraki 300-0003, Japan.
pubmed:publicationType
Journal Article