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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1998-6-4
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pubmed:abstractText |
This study describes the construction of soluble major histocompatibility complexes consisting of the mouse class I molecule, H-2Db, chemically biotinylated beta2 microglobulin and a peptide epitope derived from the glycoprotein (GP; amino acids 33-41) of lymphocytic choriomeningitis virus (LCMV). Tetrameric class I complexes, which were produced by mixing the class I complexes with phycoerythrin-labeled neutravidin, permitted direct analysis of virus-specific cytotoxic T lymphocytes (CTLs) by flow cytometry. This technique was validated by (a) staining CD8+ cells in the spleens of transgenic mice that express a T cell receptor (TCR) specific for H-2Db in association with peptide GP33-41, and (b) by staining virus-specific CTLs in the cerebrospinal fluid of C57BL/6 (B6) mice that had been infected intracranially with LCMV-DOCILE. Staining of spleen cells isolated from B6 mice revealed that up to 40% of CD8(+) T cells were GP33 tetramer+ during the initial phase of LCMV infection. In contrast, GP33 tetramers did not stain CD8+ T cells isolated from the spleens of B6 mice that had been infected 2 mo previously with LCMV above the background levels found in naive mice. The fate of virus-specific CTLs was analyzed during the acute phase of infection in mice challenged both intracranially and intravenously with a high or low dose of LCMV-DOCILE. The results of the study show that the outcome of infection by LCMV is determined by antigen load alone. Furthermore, the data indicate that deletion of virus-specific CTLs in the presence of excessive antigen is preceded by TCR downregulation and is dependent upon perforin.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-1565634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-1696684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-1699348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-1768738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-2110460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-2120065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-2199796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-2420472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-2448497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-2457647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-2468501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-2981435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-3084281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-3522223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-3871115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-3877779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-4133807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-4561841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-5097768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-6173757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-6433204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-7533855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-7753171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-7836913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-8164737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-8206875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-8344359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-8371781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-8396732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-8450214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-8469287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-8610016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-8658175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9565631-8879227
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class I,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Perforin,
http://linkedlifedata.com/resource/pubmed/chemical/Pore Forming Cytotoxic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/beta 2-Microglobulin
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
187
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1383-93
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9565631-Animals,
pubmed-meshheading:9565631-CD8-Positive T-Lymphocytes,
pubmed-meshheading:9565631-Flow Cytometry,
pubmed-meshheading:9565631-Glycoproteins,
pubmed-meshheading:9565631-Histocompatibility Antigens Class I,
pubmed-meshheading:9565631-Interferon-gamma,
pubmed-meshheading:9565631-Lymphocytic choriomeningitis virus,
pubmed-meshheading:9565631-Major Histocompatibility Complex,
pubmed-meshheading:9565631-Membrane Glycoproteins,
pubmed-meshheading:9565631-Mice,
pubmed-meshheading:9565631-Mice, Transgenic,
pubmed-meshheading:9565631-Peptides,
pubmed-meshheading:9565631-Perforin,
pubmed-meshheading:9565631-Pore Forming Cytotoxic Proteins,
pubmed-meshheading:9565631-Protein Conformation,
pubmed-meshheading:9565631-Receptors, Antigen, T-Cell,
pubmed-meshheading:9565631-Spleen,
pubmed-meshheading:9565631-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:9565631-beta 2-Microglobulin
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pubmed:year |
1998
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pubmed:articleTitle |
Induction and exhaustion of lymphocytic choriomeningitis virus-specific cytotoxic T lymphocytes visualized using soluble tetrameric major histocompatibility complex class I-peptide complexes.
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pubmed:affiliation |
Institute of Experimental Immunology, CH-8091, Zürich, Switzerland. awen.gallimore@ndm.ox.ac.uk
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