Linked Life Data

9564541

Source:http://linkedlifedata.com/resource/pubmed/id/9564541

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1998-6-5
pubmed:abstractText
We report development of a model of retroviral gene transduction in high-density limb bud cell micromass culture. The replication competent avian retrovirus RCAS BP (A) carrying the human placental alkaline phosphatase gene (RCAS AP) was used as a marker for retroviral infection and spread. The final protocol balances the need to allow time for retroviral integration and gene transduction against loss of chondrogenic potential when limb bud cells are plated at low density. It includes: (i) incubation of the dissociated limb bud cells with RCAS virus for 2 h followed by low-density culture for 48 h to allow retroviral gene expression; and (ii) secondary replating as high-density micromass culture to initiate chondrogenesis. The pattern and level of chondrogenesis in the retrovirus-transduced micromass cultures is similar to regular micromass cultures. At least 40%-50% of cells express the retroviral-transduced genes 24 h after high-density plating. This new approach facilitates ectopic gene expression in micromass culture, enabling molecular dissection of chondrogenesis and serves as a model for gene transduction in other organotypic cultures.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0736-6205
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
660-6
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Retroviral gene transfer in chondrogenic limb bud micromass cultures.
pubmed:affiliation
Department of Pathology, University Southern California, Los Angeles 90033, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't