Linked Life Data

9561560

Source:http://linkedlifedata.com/resource/pubmed/id/9561560

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-6-12
pubmed:abstractText
Recombinant Mengo viruses expressing heterologous genes have proven to be safe and immunogenic in both mice and primates, and to be able to induce both humoral and cellular immune responses (Altmeyer et al., 1995, 1996). Several recombinant Mengo viruses expressing either a large region (aa 65-206) of the HIV1 nef gene product, or cytotoxic T lymphocyte (CTL) epitopic regions from the SIV Gag (aa 182-190), Nef (aa 155-178) and Pol (aa 587-601) gene products were engineered. The heterologous antigens were expressed either as fusion proteins with the Mengo virus leader (L) protein, or in cleaved form through autocatalytic cleavage by the foot-and-mouth disease virus 2A protein. Rhesus macaques and BALB/c mice inoculated with the Mengo virus SIV recombinants failed to develop CTL responses against the SIV gene products, while one of the HIV-Nef recombinants induced a weak CTL response in mice directed to an HIV1 Nef peptide spanning positions 182-198. In contrast, BALB/c mice immunized with vaccinia virus recombinants expressing HIV1 Nef developed a strong CTL response to the 182-198 peptide and also responded to a second peptide spanning positions 73-81. These results indicate that Mengo virus recombinants expressing HIV1 Nef and SIV CTL epitopes are weak immunogens. One of the fusion recombinants expressing SIV CTL epitopes failed to infect macaques even when used at high doses, while the recombinant expressing HIV1 Nef as a fusion protein failed to infect BALB/c mice. These results demonstrate that the expression of certain heterologous sequences as fusion proteins with L can result in the loss of the ability of the recombinant to infect normally susceptible animals.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0923-2516
pubmed:author
pubmed:issnType
Print
pubmed:volume
149
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5-20
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:9561560-Amino Acid Sequence, pubmed-meshheading:9561560-Animals, pubmed-meshheading:9561560-Antibodies, Viral, pubmed-meshheading:9561560-Cytotoxicity, Immunologic, pubmed-meshheading:9561560-Epitopes, T-Lymphocyte, pubmed-meshheading:9561560-Gene Products, gag, pubmed-meshheading:9561560-Gene Products, nef, pubmed-meshheading:9561560-Gene Products, pol, pubmed-meshheading:9561560-Genetic Vectors, pubmed-meshheading:9561560-HIV Antibodies, pubmed-meshheading:9561560-HIV-1, pubmed-meshheading:9561560-HeLa Cells, pubmed-meshheading:9561560-Humans, pubmed-meshheading:9561560-Immunization, pubmed-meshheading:9561560-Macaca mulatta, pubmed-meshheading:9561560-Mengovirus, pubmed-meshheading:9561560-Mice, pubmed-meshheading:9561560-Mice, Inbred BALB C, pubmed-meshheading:9561560-Molecular Sequence Data, pubmed-meshheading:9561560-Plasmids, pubmed-meshheading:9561560-Recombinant Fusion Proteins, pubmed-meshheading:9561560-Simian immunodeficiency virus, pubmed-meshheading:9561560-T-Lymphocytes, Cytotoxic, pubmed-meshheading:9561560-Vaccinia virus, pubmed-meshheading:9561560-nef Gene Products, Human Immunodeficiency Virus
pubmed:articleTitle
Study of the immunogenicity of different recombinant Mengo viruses expressing HIV1 and SIV epitopes.
pubmed:affiliation
Unité de Virologie moléculaire, Institut Pasteur, Paris.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't