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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0021335,
umls-concept:C0025248,
umls-concept:C0042760,
umls-concept:C0079941,
umls-concept:C0206544,
umls-concept:C0456387,
umls-concept:C0599537,
umls-concept:C1171362,
umls-concept:C1515670,
umls-concept:C1883254
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pubmed:issue |
5
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pubmed:dateCreated |
1998-5-20
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pubmed:abstractText |
The bovine herpesvirus 1 (BHV-1) recombinants BHV-1/eG(ori) and BHV-1/eG(syn) were isolated after insertion of expression cassettes which contained either a genomic RNA-derived cDNA fragment (BHV-1/eG(ori)) or a modified, chemically synthesized open reading frame (ORF) (BHV-1/eG(syn)), which both encode the attachment glycoprotein G of bovine respiratory syncytial virus (BRSV), a class II membrane glycoprotein. Northern blot analyses and nuclear runoff transcription experiments indicated that transcripts encompassing the authentic BRSV G ORF were unstable in the nucleus of BHV-1/eG(ori)-infected cells. In contrast, high levels of BRSV G RNA were detected in BHV-1/eG(syn)-infected cells. Immunoblots showed that the BHV-1/eG(syn)-expressed BRSV G glycoprotein contains N- and O-linked carbohydrates and that it is incorporated into the membrane of infected cells and into the envelope of BHV-1/eG(syn) virions. The latter was also demonstrated by neutralization of BHV-1/eG(syn) infectivity by monoclonal antibodies or polyclonal anti-BRSV G antisera and complement. Our results show that expression of the BRSV G glycoprotein by BHV-1 was dependent on the modification of the BRSV G ORF and indicate that incorporation of class II membrane glycoproteins into BHV-1 virions does not necessarily require BHV-1-specific signals. This raises the possibility of targeting heterologous polypeptides to the viral envelope, which might enable the construction of BHV-1 recombinants with new biological properties and the development of improved BHV-1-based live and inactivated vector vaccines.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-1309917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-1315133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-1325699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-1371025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-1562239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-1718244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-1722936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-1847480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-1850051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-2157860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-2168991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-2214024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-2426466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-2985805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-3001696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-3027381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-3531399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-3858865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-4069997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-6090941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-6306279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-7063411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-7747461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-8077929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-8113757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-8122370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-8551568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-8627780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-8629951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-8648752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-8661416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-8683213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-8811023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-8892910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-8914247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-8985319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-9010995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-9049369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9557663-9143302
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
72
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3804-11
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9557663-Amino Acid Sequence,
pubmed-meshheading:9557663-Animals,
pubmed-meshheading:9557663-Base Sequence,
pubmed-meshheading:9557663-Cattle,
pubmed-meshheading:9557663-Cell Line,
pubmed-meshheading:9557663-DNA, Viral,
pubmed-meshheading:9557663-Gene Expression,
pubmed-meshheading:9557663-Genetic Vectors,
pubmed-meshheading:9557663-Genome, Viral,
pubmed-meshheading:9557663-Herpesvirus 1, Bovine,
pubmed-meshheading:9557663-Molecular Sequence Data,
pubmed-meshheading:9557663-Neutralization Tests,
pubmed-meshheading:9557663-Open Reading Frames,
pubmed-meshheading:9557663-Recombinant Fusion Proteins,
pubmed-meshheading:9557663-Recombination, Genetic,
pubmed-meshheading:9557663-Respiratory Syncytial Virus, Bovine,
pubmed-meshheading:9557663-Transcription, Genetic,
pubmed-meshheading:9557663-Viral Envelope Proteins,
pubmed-meshheading:9557663-Virion
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pubmed:year |
1998
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pubmed:articleTitle |
The class II membrane glycoprotein G of bovine respiratory syncytial virus, expressed from a synthetic open reading frame, is incorporated into virions of recombinant bovine herpesvirus 1.
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