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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1998-5-28
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pubmed:abstractText |
The existence of an activity corresponding to the nucleotide-induced Yeast Mitochondria Unselective Channel (YMUC2) of isolated mitochondria was investigated in permeabilized and intact spheroplasts of the baker's yeast Yeast Foam. In nystatin-permeabilized spheroplasts, ATP and GDP-beta-S induced a decavanadate-sensitive stimulation of the respiration only under conditions equivalent to those previously reported for isolated mitochondria (low phosphate concentration, presence of a salt). On intact spheroplasts parallel measurements of respiration rate, [ATP]/[ADP] ratio and mitochondrial transmembrane potential allowed to show that the addition of the glucose analog 2-deoxyglucose decreased the permeability of the inner mitochondrial membrane owing to cellular ATP depletion. This strongly supports the hypothesis that Yeast Mitochondria Unspecific Channel is active in situ and inhibited by cellular [ATP] depletion.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Diphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Carbonyl Cyanide m-Chlorophenyl...,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyglucose,
http://linkedlifedata.com/resource/pubmed/chemical/Ion Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Lactic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/NAD,
http://linkedlifedata.com/resource/pubmed/chemical/Nystatin,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Sorbitol,
http://linkedlifedata.com/resource/pubmed/chemical/Triethyltin Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/triethyltin
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1039-9712
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
44
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
565-75
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9556218-Adenosine Diphosphate,
pubmed-meshheading:9556218-Adenosine Triphosphate,
pubmed-meshheading:9556218-Carbonyl Cyanide m-Chlorophenyl Hydrazone,
pubmed-meshheading:9556218-Cell Membrane Permeability,
pubmed-meshheading:9556218-Cell Respiration,
pubmed-meshheading:9556218-Deoxyglucose,
pubmed-meshheading:9556218-Ion Channels,
pubmed-meshheading:9556218-Lactic Acid,
pubmed-meshheading:9556218-Mitochondria,
pubmed-meshheading:9556218-NAD,
pubmed-meshheading:9556218-Nystatin,
pubmed-meshheading:9556218-Phosphates,
pubmed-meshheading:9556218-Saccharomyces cerevisiae,
pubmed-meshheading:9556218-Sorbitol,
pubmed-meshheading:9556218-Spheroplasts,
pubmed-meshheading:9556218-Triethyltin Compounds
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pubmed:year |
1998
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pubmed:articleTitle |
Investigation of the yeast mitochondrial unselective channel in intact and permeabilized spheroplasts.
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pubmed:affiliation |
Institut de Biochimie et de Génétique Cellulaires du Centre National de la Recherche Scientifique, Université Victor Ségalen-Bordeaux II, France. stephen.manon@ibgc.u-bordeaux2.fr
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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