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rdf:type |
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lifeskim:mentions |
umls-concept:C0007578,
umls-concept:C0014257,
umls-concept:C0016030,
umls-concept:C0026473,
umls-concept:C0035820,
umls-concept:C0054942,
umls-concept:C0064830,
umls-concept:C0078056,
umls-concept:C0086597,
umls-concept:C0173022,
umls-concept:C0205087,
umls-concept:C0209606,
umls-concept:C0298340,
umls-concept:C0441712,
umls-concept:C0600210,
umls-concept:C1416487,
umls-concept:C1416488,
umls-concept:C1416496,
umls-concept:C1531430,
umls-concept:C1550315
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pubmed:issue |
1
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pubmed:dateCreated |
1998-5-7
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pubmed:abstractText |
Monocytes migrate through vascular endothelium, and then in connective tissue. As a model of this process, we investigated adhesion molecules involved in monocyte migration through HUVEC and a barrier of human synovial fibroblasts (HSF). Minimal spontaneous monocyte migration (6-7%) occurred through either cell barrier, but this increased markedly (27-35% of added monocytes) when a C5a chemotactic gradient was present. Migration across unstimulated HUVEC was partially inhibited (40%) by mAb to CD18 (beta2 integrin) and completely blocked by anti-CD18 plus anti-alpha4 (CD49d; very late Ag-4 (VLA-4)) mAbs. In contrast, migration across HSF induced by C5a or monocyte chemoattractant protein-1 was not inhibited by mAb to CD18 and was only partially inhibited (33%) in combination with anti-alpha4 mAb. The CD18- and VLA-4-independent migration across HSF was completely inhibited by mAb to alpha5 of VLA-5. The inhibitory effect of mAbs to VLA-4 and VLA-5 was on the monocyte and required blockade of CD11/CD18 to be observed. In contrast to HSF, no role for VLA-5 in monocyte transendothelial migration was detected. Both HSF and IL-1-stimulated HUVEC expressed vascular cell adhesion molecule-1 (VCAM-1). However, VLA-4-mediated monocyte migration across HSF was only partially dependent on VCAM-1, in contrast to transendothelial migration, which was completely blocked by anti-VCAM-1 mAbs. In conclusion, unlike transendothelial migration, for which VLA-4 is the alternative mechanism to CD11/CD18 on monocytes, both VLA-4 and VLA-5 can mediate monocyte migration through fibroblast barriers. In addition to VCAM-1, other ligand(s) on HSF are also involved in the VLA-4-mediated migration.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD11,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD18,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL2,
http://linkedlifedata.com/resource/pubmed/chemical/Complement C5a,
http://linkedlifedata.com/resource/pubmed/chemical/Integrin alpha4beta1,
http://linkedlifedata.com/resource/pubmed/chemical/Integrin alpha6beta1,
http://linkedlifedata.com/resource/pubmed/chemical/Integrins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Fibronectin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Lymphocyte Homing,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Cell Adhesion Molecule-1
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
160
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
467-74
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9552005-Antibodies, Monoclonal,
pubmed-meshheading:9552005-Antigens, CD11,
pubmed-meshheading:9552005-Antigens, CD18,
pubmed-meshheading:9552005-Cell Adhesion Molecules,
pubmed-meshheading:9552005-Cell Movement,
pubmed-meshheading:9552005-Chemokine CCL2,
pubmed-meshheading:9552005-Complement C5a,
pubmed-meshheading:9552005-Endothelium,
pubmed-meshheading:9552005-Fibroblasts,
pubmed-meshheading:9552005-Humans,
pubmed-meshheading:9552005-Immunologic Techniques,
pubmed-meshheading:9552005-Integrin alpha4beta1,
pubmed-meshheading:9552005-Integrin alpha6beta1,
pubmed-meshheading:9552005-Integrins,
pubmed-meshheading:9552005-Monocytes,
pubmed-meshheading:9552005-Receptors, Fibronectin,
pubmed-meshheading:9552005-Receptors, Lymphocyte Homing,
pubmed-meshheading:9552005-Synovial Membrane,
pubmed-meshheading:9552005-Vascular Cell Adhesion Molecule-1
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pubmed:year |
1998
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pubmed:articleTitle |
Adhesion molecule mechanisms mediating monocyte migration through synovial fibroblast and endothelium barriers: role for CD11/CD18, very late antigen-4 (CD49d/CD29), very late antigen-5 (CD49e/CD29), and vascular cell adhesion molecule-1 (CD106).
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pubmed:affiliation |
Department of Pediatrics, Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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