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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1998-5-27
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pubmed:abstractText |
The high ligation specificity of a tetranucleotide with a pair of flanking octanucleotides on DNA template by the action of T4 phage DNA ligase is shown. In a tetranucleotide-DNA template complex containing a mismatch, almost no ligation products are formed. The ligation of a tandem octanucleotide-tetranucleotide-octanucleotide makes it possible to identify accurately any single nucleotide substitution in a tetranucleotide binding site.
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pubmed:language |
rus
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
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pubmed:issn |
0132-3423
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
|
pubmed:pagination |
32-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9551199-Autoradiography,
pubmed-meshheading:9551199-Binding Sites,
pubmed-meshheading:9551199-DNA,
pubmed-meshheading:9551199-DNA Ligases,
pubmed-meshheading:9551199-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9551199-Oligonucleotides,
pubmed-meshheading:9551199-Point Mutation,
pubmed-meshheading:9551199-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:9551199-Temperature,
pubmed-meshheading:9551199-Templates, Genetic
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pubmed:year |
1998
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pubmed:articleTitle |
[Interaction of short oligonucleotides derivatives with nucleic acids. VI. Discrimination of mismatch-containing complexes upon ligation of a short oligonucleotide tandem on DNA template].
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pubmed:affiliation |
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, Novosibirsk, Russia.
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pubmed:publicationType |
Journal Article,
English Abstract
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