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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1998-7-16
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pubmed:abstractText |
1. Ionic currents were simultaneously recorded at macroscopic and unitary level using the whole-cell and cell-attached patch-clamp procedures together on the same portion of isolated mouse skeletal muscle fibres. 2. In the presence of Tyrode solution in the patch pipette and Tyrode-TTX solution in the bath, macroscopic and unitary currents through delayed rectifier K+ channels were simultaneously recorded in response to depolarizing pulses of 1 s duration. 3. In five fibres, successive long-lasting incremental depolarizing levels induced, at -40 mV or -30 mV, the opening of a high conductance channel carrying an outward current superimposed on delayed rectifier K+ channel activity. Opening of this high conductance channel was not observed when the depolarization steps were applied in the patch pipette. 4. Using the same depolarizing protocol, activation of a high conductance channel was also observed in two fibres in the presence of a K+-rich solution in the pipette (145 mM K+) . 5. With either Tyrode or K+-rich solution in the pipette, unitary current amplitudes of the high conductance channel matched well with the values obtained for Ca2+-activated K+ (KCa) channels in inside-out patches under similar ionic conditions. 6. Indo-1 fluorescence measurements showed that the stimulation protocol that led to KCa channel opening induced stepwise increases in intracellular [Ca2+] in the submicromolar range. 7. Our results provide evidence that activation of sarcolemmal KCa channels can be induced by a rise in intracellular [Ca2+] following voltage-activated sarcoplasmic reticulum Ca2+ release.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9547384-13714849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9547384-1761971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9547384-1917908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9547384-2391651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9547384-2479142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9547384-2653189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9547384-3838314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9547384-4600885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9547384-6296366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9547384-6304231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9547384-671323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9547384-8735698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9547384-8807400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9547384-8841995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9547384-9251808
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
509 ( Pt 1)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
93-102
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9547384-Animals,
pubmed-meshheading:9547384-Biotransformation,
pubmed-meshheading:9547384-Calcium,
pubmed-meshheading:9547384-Electric Stimulation,
pubmed-meshheading:9547384-Electrophysiology,
pubmed-meshheading:9547384-Fluorescent Dyes,
pubmed-meshheading:9547384-Indoles,
pubmed-meshheading:9547384-Male,
pubmed-meshheading:9547384-Membrane Potentials,
pubmed-meshheading:9547384-Mice,
pubmed-meshheading:9547384-Muscle, Skeletal,
pubmed-meshheading:9547384-Muscle Fibers, Skeletal,
pubmed-meshheading:9547384-Patch-Clamp Techniques,
pubmed-meshheading:9547384-Potassium Channels,
pubmed-meshheading:9547384-Sarcolemma,
pubmed-meshheading:9547384-Tetrodotoxin
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pubmed:year |
1998
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pubmed:articleTitle |
Activation of Ca2+-activated K+ channels by an increase in intracellular Ca2+ induced by depolarization of mouse skeletal muscle fibres.
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pubmed:affiliation |
Laboratoire de Physiologie des Elements Excitables, CNRS UMR 5578, Universite Claude Bernard Lyon 1, 43 boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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