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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1998-7-6
|
| pubmed:abstractText |
RBM is a germ-cell-specific RNA-binding protein encoded by the Y chromosome in all mammals, implying an important and evolutionarily conserved (but as yet unidentified) function during male germ cell development. In order to address this function, we have developed new antibody reagents to immunolocalise RBM in the different cell types in the human testis. We find that RBM has a different expression profile from its closest homologue hnRNPG. Despite its ubiquitous expression in all transcriptionally active germ cell types, RBM has a complex and dynamic cell biology in human germ cells. The ratio of RBM distributed between punctate nuclear structures and the remainder of the nucleoplasm is dynamically modulated over the course of germ cell development. Moreover, pre-mRNA splicing components are targeted to the same punctate nuclear regions as RBM during the early stages of germ cell development but late in meiosis this spatial association breaks down. After meiosis, pre-mRNA splicing components are differentially targeted to a specific region of the nucleus. While pre-mRNA splicing components undergo profound spatial reorganisations during spermatogenesis, neither heterogeneous ribonucleoproteins nor the transcription factor Sp1 show either developmental spatial reorganisations or any specific co-localisation with RBM. These results suggest dynamic and possibly multiple functions for RBM in germ cell development.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response Element...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Heterogeneous-Nuclear...,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RBMY1A1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sp1 Transcription Factor
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0021-9533
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
111 ( Pt 9)
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1255-65
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9547301-Cyclic AMP Response Element Modulator,
pubmed-meshheading:9547301-DNA-Binding Proteins,
pubmed-meshheading:9547301-Heterogeneous-Nuclear Ribonucleoproteins,
pubmed-meshheading:9547301-Humans,
pubmed-meshheading:9547301-Male,
pubmed-meshheading:9547301-Meiosis,
pubmed-meshheading:9547301-Nuclear Proteins,
pubmed-meshheading:9547301-Prostate,
pubmed-meshheading:9547301-RNA,
pubmed-meshheading:9547301-RNA Precursors,
pubmed-meshheading:9547301-RNA Splicing,
pubmed-meshheading:9547301-RNA-Binding Proteins,
pubmed-meshheading:9547301-Repressor Proteins,
pubmed-meshheading:9547301-Ribonucleoproteins,
pubmed-meshheading:9547301-Sp1 Transcription Factor,
pubmed-meshheading:9547301-Spermatogenesis,
pubmed-meshheading:9547301-Spermatozoa,
pubmed-meshheading:9547301-Testis
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Dynamic changes in the subnuclear organisation of pre-mRNA splicing proteins and RBM during human germ cell development.
|
| pubmed:affiliation |
Medical Research Council Genetics Unit, Western General Hospital, Edinburgh, Scotland. davide@hgu.mrc.ac.uk
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|