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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1998-4-16
|
| pubmed:abstractText |
The hypothesis that a low concordance rate in monozygotic (MZ) twins with systemic lupus erythematosus (SLE) may be accounted for by differences in X-chromosome inactivation was examined. Five MZ twin pairs, four discordant and one concordant, were recruited, zygosity confirmed by DNA fingerprinting, and their pattern of X-chromosome inactivation in DNA samples prepared from peripheral blood and buccal cells were examined. X-chromosome inactivation was assessed by the methylation status of the CpG region near trinucleotide repeats in exon 1 of the androgen receptor gene on X-chromosome after digestion with the methylation-sensitive enzyme HpaII or HhaI and PCR amplification. X-chromosome inactivation patterns were found to be the same between affected and non-affected twins in all four discordant twin pairs, with random patterns in two pairs and skewed patterns in the others. The concordant twins demonstrated the same random patterns. X-chromosome inactivation was also examined from buccal smear DNA and shown to have the same pattern as that noted from peripheral blood DNA in one informative twin pair. Differences in X-chromosome inactivation patterns were not observed in these five MZ twin pairs. The results could not support the hypothesis that differences in X-chromosome inactivation is the mechanism accounting for the low concordance rate noted in MZ twins with SLE.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0891-6934
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
26
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
85-93
|
| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:9546817-Adult,
pubmed-meshheading:9546817-Aged,
pubmed-meshheading:9546817-Antibodies, Antinuclear,
pubmed-meshheading:9546817-Cheek,
pubmed-meshheading:9546817-DNA,
pubmed-meshheading:9546817-DNA Fingerprinting,
pubmed-meshheading:9546817-DNA-Cytosine Methylases,
pubmed-meshheading:9546817-Diseases in Twins,
pubmed-meshheading:9546817-Dosage Compensation, Genetic,
pubmed-meshheading:9546817-Electrophoresis, Agar Gel,
pubmed-meshheading:9546817-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9546817-Female,
pubmed-meshheading:9546817-Genetic Linkage,
pubmed-meshheading:9546817-Humans,
pubmed-meshheading:9546817-Lupus Erythematosus, Systemic,
pubmed-meshheading:9546817-Male,
pubmed-meshheading:9546817-Microsatellite Repeats,
pubmed-meshheading:9546817-Middle Aged,
pubmed-meshheading:9546817-Minisatellite Repeats,
pubmed-meshheading:9546817-Polymerase Chain Reaction,
pubmed-meshheading:9546817-Twins, Monozygotic,
pubmed-meshheading:9546817-X Chromosome
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
X-chromosome inactivation in monozygotic twins with systemic lupus erythematosus.
|
| pubmed:affiliation |
Department of Rheumatology, Royal North Shore Hospital St. Leonards, NSW, Australia.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Twin Study
|