Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1998-5-1
pubmed:databankReference
pubmed:abstractText
A 6.0-kb SalI DNA fragment containing an entire rRNA operon (rrnB) was cloned from a cosmid gene bank of the phytopathogenic strain Rhodococcus fascians D188. The nucleotide sequence of the 6-kb fragment was determined and had the organization 16S rRNA-spacer-23S rRNA-spacer-5S rRNA without tRNA-encoding genes in the spacer regions. The 5' and 3' ends of the mature 16S, 23S, and 5S rRNAs were determined by alignment with the rrn operons of Bacillus subtilis and other gram-positive bacteria. Four copies of the rrn operons were identified by hybridization with an rrnB probe in R. fascians type strain ATCC 12974 and in the virulent strain R. fascians D188. However, another isolate, CECT 3001 (= NRRL B15096), also classified as R. fascians, produced five rrn-hybridizing bands. An integrative vector containing a 2.5-kb DNA fragment internal to rrnB was constructed for targeted integration of exogenous genes at the rrn loci. Transformants carrying the exogenous chloramphenicol resistance gene (cmr) integrated in different rrn operons were obtained. These transformants had normal growth rates in complex medium and minimal medium and were fully stable for the integrated marker.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-1326592, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-1367217, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-1396599, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-1406276, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-1547783, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-1662759, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-2414156, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-2762130, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-3162908, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-3729405, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-388356, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-6162835, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-6345791, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-6756296, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-7028991, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-7533593, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-7608093, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-7693070, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-8169198, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-8226696, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-8341713, http://linkedlifedata.com/resource/pubmed/commentcorrection/9546162-8842145
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1276-82
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:9546162-Base Sequence, pubmed-meshheading:9546162-Cloning, Molecular, pubmed-meshheading:9546162-DNA, Bacterial, pubmed-meshheading:9546162-DNA, Ribosomal, pubmed-meshheading:9546162-Gene Targeting, pubmed-meshheading:9546162-Genes, Bacterial, pubmed-meshheading:9546162-Genetic Vectors, pubmed-meshheading:9546162-Molecular Sequence Data, pubmed-meshheading:9546162-Operon, pubmed-meshheading:9546162-Plants, pubmed-meshheading:9546162-RNA, Bacterial, pubmed-meshheading:9546162-RNA, Ribosomal, 16S, pubmed-meshheading:9546162-RNA, Ribosomal, 23S, pubmed-meshheading:9546162-RNA, Ribosomal, 5S, pubmed-meshheading:9546162-Restriction Mapping, pubmed-meshheading:9546162-Rhodococcus, pubmed-meshheading:9546162-Sequence Homology, Nucleic Acid, pubmed-meshheading:9546162-Virulence
pubmed:year
1998
pubmed:articleTitle
Characterization of the rrnB operon of the plant pathogen Rhodococcus fascians and targeted integrations of exogenous genes at rrn loci.
pubmed:affiliation
Department of Ecology, Genetics and Microbiology, Faculty of Biology, University of León, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't