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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
16
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pubmed:dateCreated |
1998-5-21
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pubmed:abstractText |
During keratinocyte differentiation, the glycolipid, glucosylceramide (GlcCer), is thought to be synthesized, stored in intracellular lamellar granules and eventually extruded into the intercellular space where GlcCer is hydrolyzed to ceramide, a major component of the epidermal permeability barrier. Previous studies showed that GlcCer synthase (GCS) activity increases during keratinocyte differentiation; however, the mechanism by which GCS activity is regulated was not established. In the present study, we prepared anti-peptide antibodies and amplified cDNA probes based on the cDNA sequence for human GCS (Ichikawa, S., Sakiyama, H., Suzuki, G., Hidari, K. I.-P. J., and Hirabayashi, Y. (1996) Proc. Natl. Acad. Sci. U. S. A. 93, 4638-4643) in order to study GCS expression during keratinocyte differentiation. Confluent human keratinocytes in culture were induced to terminally differentiate by elevation of Ca+2 in the medium without exogenous hormones or growth factors. GlcCer synthesis assayed in situ using a fluorescent ceramide analog increased approximately 5-fold during keratinocyte differentiation, peaking at day 6. Fluorescence microscopy studies of living keratinocytes showed that fluorescent ceramide and/or its metabolites accumulated in the Golgi in undifferentiated cells but targeted to unique vesicular structures that may be derived from the trans-Golgi region. Expression of both GCS mRNA, a approximately 3. 8-kilobase transcript on Northern blots, and GCS protein, a approximately 38-kDa polypeptide detected by Western blotting, increased dramatically (approximately 5-fold) during differentiation, reaching a maximum at about day 8. These results suggest that GCS is up-regulated at the transcriptional level during keratinocyte differentiation and provide the first direct evidence for GCS up-regulation in any cell type.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Glucosyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/ceramide glucosyltransferase
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9651-5
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9545298-Amino Acid Sequence,
pubmed-meshheading:9545298-Antibodies,
pubmed-meshheading:9545298-Calcium,
pubmed-meshheading:9545298-Cell Differentiation,
pubmed-meshheading:9545298-Cells, Cultured,
pubmed-meshheading:9545298-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:9545298-Glucosyltransferases,
pubmed-meshheading:9545298-Humans,
pubmed-meshheading:9545298-Infant, Newborn,
pubmed-meshheading:9545298-Keratinocytes,
pubmed-meshheading:9545298-Kinetics,
pubmed-meshheading:9545298-Male,
pubmed-meshheading:9545298-Molecular Sequence Data,
pubmed-meshheading:9545298-Peptide Fragments,
pubmed-meshheading:9545298-Protein Biosynthesis,
pubmed-meshheading:9545298-RNA, Messenger,
pubmed-meshheading:9545298-Skin,
pubmed-meshheading:9545298-Time Factors,
pubmed-meshheading:9545298-Transcription, Genetic
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pubmed:year |
1998
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pubmed:articleTitle |
Up-regulation of glucosylceramide synthase expression and activity during human keratinocyte differentiation.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Thoracic Disease Research Unit, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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