Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1998-6-24
pubmed:databankReference
pubmed:abstractText
Ubiquitin conjugation is known to target protein substrates primarily to degradation by the proteasome or via the endocytic route. Here we describe a novel protein modification pathway in yeast which mediates the conjugation of RUB1, a ubiquitin-like protein displaying 53% amino acid identity to ubiquitin. We show that RUB1 conjugation requires at least three proteins in vivo. ULA1 and UBA3 are related to the N- and C-terminal domains of the E1 ubiquitin-activating enzyme, respectively, and together fulfil E1-like functions for RUB1 activation. RUB1 conjugation also requires UBC12, a protein related to E2 ubiquitin-conjugating enzymes, which functions analogously to E2 enzymes in RUB1-protein conjugate formation. Conjugation of RUB1 is not essential for normal cell growth and appears to be selective for a small set of substrates. Remarkably, CDC53/cullin, a common subunit of the multifunctional SCF ubiquitin ligase, was found to be a major substrate for RUB1 conjugation. This suggests that the RUB1 conjugation pathway is functionally affiliated to the ubiquitin-proteasome system and may play a regulatory role.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-1336336, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-1373138, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-1378265, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-17708991, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-1989885, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-2154373, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-2193438, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-2440890, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-3030556, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-3038523, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-3041007, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-3053175, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-3073106, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-7526157, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-7553848, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-7629121, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-7800044, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-7834747, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-7883165, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-7923371, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-7954792, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-8045256, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-8204681, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-8221889, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-8246991, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-8565073, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-8631913, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-8681378, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-8682868, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-8756727, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-8943317, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-8978815, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-8982460, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-9019411, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-9305631, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-9312007, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-9312010, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-9312022, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-9312043, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-9321400, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-9341106, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-9346238, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-9346239, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-9353319, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-9357311, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-9357313, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-9427648, http://linkedlifedata.com/resource/pubmed/commentcorrection/9545234-9435231
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Cdc53 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Cullin Proteins, http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary, http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Ligases, http://linkedlifedata.com/resource/pubmed/chemical/RUB1 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins, http://linkedlifedata.com/resource/pubmed/chemical/ULA1 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin-Activating Enzymes, http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin-Conjugating Enzymes, http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin-Protein Ligases, http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitins
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2208-14
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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