Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1998-5-20
|
| pubmed:abstractText |
Enzyme immunoassay (EIA) is an ideal method for screening large numbers of patients for syphilis. We evaluated a novel immune-capture EIA (ICE Syphilis; Murex Diagnostics) that uses three recombinant Treponema pallidum antigens (TpN15, TpN17, and TpN47) and compared the results with those obtained by the native T. pallidum antigen EIA (Captia SelectSyph-G; Centocor) that we currently use for the serodiagnosis of syphilis. Specificity was evaluated by screening 1,184 unselected serum specimens in parallel by the ICE Syphilis and SelectSyph-G assays, while sensitivity was tested with a panel of 101 serum specimens containing antitreponemal antibodies (treated and untreated) from patients with various stages of infection. The specificity of the ICE Syphilis EIA (99.8%) on screening was significantly higher (P < 0.02) than that of the SelectSyph-G EIA (99.2%). The sensitivity of the ICE Syphilis EIA was significantly higher (P < 0.01) than that of the SelectSyph-G EIA on both initial (99 versus 91.4%) and repeat (100 versus 92.4%) testing. The ICE Syphilis EIA was also significantly more sensitive (P < 0.01) than the fluorescent treponemal antibody-abs (92.4%) but not the T. pallidum hemagglutination assay (97.1%). Sera containing antitreponemal antibodies gave a much higher antibody index (absorbance of test serum/kit cutoff) by the ICE Syphilis EIA than by the SelectSyph-G EIA. This combined with the overall high sensitivity makes the ICE Syphilis EIA an ideal test for excluding or detecting treponemal infection in human immunodeficiency virus (HIV)-infected patients. The ICE Syphilis EIA was positive with sera from all 15 HIV-infected patients in the study, whereas sera from 3 HIV-infected patients were negative by the SelectSyph-G EIA. We conclude that the high sensitivity and specificity of the ICE Syphilis EIA and its suitability for automation make it an ideal screening test.
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9542908-1286115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9542908-1740512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9542908-1976130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9542908-2059384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9542908-2403176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9542908-2643617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9542908-2963840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9542908-3056164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9542908-3316921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9542908-3881539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9542908-7604648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9542908-7704889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9542908-7751351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9542908-7779920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9542908-7832818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9542908-7969296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9542908-8068145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9542908-8890687
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0095-1137
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
36
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
913-7
|
| pubmed:dateRevised |
2009-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:9542908-Antigens, Bacterial,
pubmed-meshheading:9542908-HIV Infections,
pubmed-meshheading:9542908-Humans,
pubmed-meshheading:9542908-Immunoenzyme Techniques,
pubmed-meshheading:9542908-Recombinant Proteins,
pubmed-meshheading:9542908-Sensitivity and Specificity,
pubmed-meshheading:9542908-Syphilis Serodiagnosis,
pubmed-meshheading:9542908-Treponema pallidum
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Novel recombinant-antigen enzyme immunoassay for serological diagnosis of syphilis.
|
| pubmed:affiliation |
Department of Medical Microbiology, Edinburgh University Medical School, Scotland, United Kingdom. hugh.young@ed.ac.uk
|
| pubmed:publicationType |
Journal Article
|