Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1998-5-21
|
| pubmed:abstractText |
The mechanisms behind the effects of immunotherapy (IT) with birch-pollen extract are largely unknown. In this pilot study, we measured the cytokine secretion in vitro from peripheral blood mononuclear cells (PBMC) obtained from birch-pollen-allergic patients undergoing IT treatment (n = 4) or placebo administration (n = 4), collected before treatment, 1 and 4 weeks after start of treatment, and during and just after the pollen season (12-14 weeks after start of treatment). The PBMC were stimulated with birch-pollen extract in vitro for 7 days, followed by restimulation with the mitogens phytohemagglutinin (PHA) and phorbol 12-myristate 13-acetate (PMA) for 24 h, to enhance the production of cytokines. The supernatants were analyzed with ELISA and radioimmunoassay for interferon-gamma (IFN-gamma), interleukin (IL)-4, and IL-5. In the therapy group, we noted an increased secretion of IL-4 and IL-5 from PBMC collected at 4 weeks after the start of treatment (IL-4: 29 +/- 21 pg/ml [day 0] to 374 +/- 448 pg/ml [week 4], mean +/- SD; IL-5: 95 +/- 48 pg/ml to 1147 +/- 697 pg/ml). No increase was seen in the placebo group. During the pollen season, we noted a trend toward increased IL-4 and IL-5 secretion in both groups. We conclude that the temporary increase in serum IgE observed in many IT studies may be a consequence of increased IL-4 production due to the allergen exposure.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Allergens,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-5
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0105-4538
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
53
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
275-81
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9542607-Adult,
pubmed-meshheading:9542607-Allergens,
pubmed-meshheading:9542607-Asthma,
pubmed-meshheading:9542607-Cells, Cultured,
pubmed-meshheading:9542607-Desensitization, Immunologic,
pubmed-meshheading:9542607-Double-Blind Method,
pubmed-meshheading:9542607-Female,
pubmed-meshheading:9542607-Humans,
pubmed-meshheading:9542607-Immunoglobulin E,
pubmed-meshheading:9542607-Immunoglobulin G,
pubmed-meshheading:9542607-Interferon-gamma,
pubmed-meshheading:9542607-Interleukin-4,
pubmed-meshheading:9542607-Interleukin-5,
pubmed-meshheading:9542607-Leukocytes, Mononuclear,
pubmed-meshheading:9542607-Lymphocyte Activation,
pubmed-meshheading:9542607-Male,
pubmed-meshheading:9542607-Middle Aged,
pubmed-meshheading:9542607-Pilot Projects,
pubmed-meshheading:9542607-Pollen,
pubmed-meshheading:9542607-Rhinitis, Allergic, Seasonal
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Allergen-specific increase in interleukin (IL)-4 and IL-5 secretion from peripheral blood mononuclear cells during birch-pollen immunotherapy.
|
| pubmed:affiliation |
Department of Asthma/Allergy, Pharmacia & Upjohn Diagnostics AB, Uppsala, Sweden.
|
| pubmed:publicationType |
Journal Article,
Clinical Trial,
Randomized Controlled Trial
|