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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1998-4-23
|
| pubmed:abstractText |
We previously reported the expression of soluble A/Victoria/3/75 (H3N2) hemagglutinin in insect cells and the molecular and immunological structure of an aggregated fraction, only observed in cell supernatant when expression was performed at low pH [23]. Here we report that besides this aggregated a monomeric and possibly a trimeric structure is detected in cell supernatant, irrespective of the pH of the medium. Evidence is presented that the aggregated fraction is generated out of monomeric HAOs molecules due to a low intracellular pH encountered during secretion.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0304-8608
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
143
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
227-39
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9541609-Animals,
pubmed-meshheading:9541609-Antibodies, Monoclonal,
pubmed-meshheading:9541609-Cell Line,
pubmed-meshheading:9541609-Hemagglutinin Glycoproteins, Influenza Virus,
pubmed-meshheading:9541609-Hydrogen-Ion Concentration,
pubmed-meshheading:9541609-Protein Folding,
pubmed-meshheading:9541609-Recombinant Proteins,
pubmed-meshheading:9541609-Spodoptera
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
pH-dependent aggregation and secretion of soluble monomeric influenza hemagglutinin.
|
| pubmed:affiliation |
Laboratory of Molecular Biology, Flanders Interuniversity Institute of Biotechnology, University of Ghent, Belgium.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|