Linked Life Data

9541462

Source:http://linkedlifedata.com/resource/pubmed/id/9541462

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-5-26
pubmed:abstractText
Dysregulation of cytokine production in atopic individuals has previously been clearly demonstrated. In the present study we aimed to assess whether a prolonged in vitro exposure of peripheral blood lymphocytes (PBMC) to allergen would result in a, by time, changed cytokine profile in allergic subjects. Blood was taken from 11 atopic asthmatic subjects and nine healthy non-atopic controls during the birch pollen season. PBMC were stimulated with birch pollen allergen (100, 1000 and 10,000 SQ-U/ml). After different times of exposure (1, 3 and 5 weeks) interleukin (IL)-5 and interferon-gamma (IFN-gamma) production was measured. Prior to the IL5 and IFN-gamma determinations, PHA was added to the cultures to ensure maximal release of cytokines. The asthmatic group always displayed a lower IFN-gamma:IL-5 ratio. Significant differences in ratio between the two groups were observed. Furthermore, PBMC challenged in vitro with 10,000 SQ-U/ml resulted in significantly elevated levels of IL-5 in the asthmatic group compared to the control group when determined after 3 and 5 weeks stimulation. In the asthmatic group the balance between IL-5 and IFN-gamma is shifted towards increased IL-5 production following prolonged in vitro stimulation. This might illustrate the in vivo situation, where an increased IL-5 production is of importance in the pathogenesis of asthma.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0165-2478
pubmed:author
pubmed:issnType
Print
pubmed:volume
60
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
45-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Cytokine production in PBMC from allergics and non-allergics following in vitro allergen stimulation.
pubmed:affiliation
Department of Laboratory Medicine, Karolinska Hospital, Stockholm, Sweden.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't