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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1998-4-20
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pubmed:abstractText |
Increasing evidence suggests that cytokines may play a role in ovarian processes. The purpose of this study was to determine if interleukin-1 (IL-1) could modulate rabbit pre-ovulatory follicular function and to explore cellular sites of IL-1 biosynthesis in rabbit follicles. Development of rabbit pre-ovulatory follicles was induced by 200 mIU equine chorionic gonadotropin daily for 2 days. Seventy-two hours after the last injection, ovaries were excised and granulosa and theca cells isolated. The two types of cell were pre-incubated for 24 h in Minimum Essential Medium (MEM) with 5% fetal calf serum (FCS), and then incubated for 24 h in MEM with 2.5% FCS with appropriate stimulants. Results showed that rabbit granulosa and theca cell culture supernatants contain IL-1 bioactivity and that this bioactivity was diminished by gonadotropins, FSH and human chorionic gonadotropin, in a dose-dependent manner. Low doses of IL-1 (1 ng/ml) inhibited gonadotropin-induced progesterone production in both cell types and at the same time increased cell numbers. A study of the mechanism of IL-1 action demonstrated that it affects cAMP generation, and also steps distal to cAMP formation. We conclude that in our model gonadotropins, by inhibiting IL-1 production, could control IL-1 modulation of gonadotropin action on steroidogenesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0804-4643
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
138
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
328-36
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9539309-1-Methyl-3-isobutylxanthine,
pubmed-meshheading:9539309-Animals,
pubmed-meshheading:9539309-Bucladesine,
pubmed-meshheading:9539309-Cell Division,
pubmed-meshheading:9539309-Cells, Cultured,
pubmed-meshheading:9539309-Cholera Toxin,
pubmed-meshheading:9539309-Chorionic Gonadotropin,
pubmed-meshheading:9539309-Cyclic AMP,
pubmed-meshheading:9539309-Dose-Response Relationship, Drug,
pubmed-meshheading:9539309-Female,
pubmed-meshheading:9539309-Follicle Stimulating Hormone,
pubmed-meshheading:9539309-Forskolin,
pubmed-meshheading:9539309-Granulosa Cells,
pubmed-meshheading:9539309-Humans,
pubmed-meshheading:9539309-Interleukin-1,
pubmed-meshheading:9539309-Mice,
pubmed-meshheading:9539309-Phosphodiesterase Inhibitors,
pubmed-meshheading:9539309-Progesterone,
pubmed-meshheading:9539309-Rabbits,
pubmed-meshheading:9539309-Theca Cells
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pubmed:year |
1998
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pubmed:articleTitle |
Inhibition by gonadotropins of interleukin-1 production by rabbit granulosa and theca cells: effects on gonadotropin-induced progesterone production.
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pubmed:affiliation |
Laboratoire de Biochimie, EP 9 CNRS, IBBA Université de Caen, CHU C ote de Nacre, France.
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pubmed:publicationType |
Journal Article,
Comparative Study
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