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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1998-5-26
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pubmed:abstractText |
Although NGF (nerve growth factor) induces neuronal differentiation of PC12 cells, EGF (epidermal growth factor) acts as a mitogen for these cells. We have studied the effects of a synthetic pyrimidine derivative, MS-430, on the NGF and EGF actions on PC12h cells. We found that MS-430 accelerated NGF-induced neurite extension of PC12h cells and that, in the presence of MS-430, PC12h cells extended neurites in response to EGF. Next, we investigated the tyrosine phosphorylation of NGF receptor TrkA and the EGF receptor (EGFR) as well as mitogen-activated protein kinase (MAPK), which is a key protein in the intracellular signal transduction pathway. It was found that MS-430 prolonged the EGF-induced phosphorylation of EGFR and MAPK compared to that without MS-430. MS-430 also prolonged the NGF-induced phosphorylation of MAPK, but the phosphorylation of TrkA induced by NGF was not affected by MS-430. These results suggest that MS-430 influences the intracellular signal transduction pathway which causes the neuronal differentiation of PC12h cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholinesterase,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/MS 430,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrimidines,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, trkA,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Nerve Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-924X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
123
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
423-30
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9538224-Acetylcholinesterase,
pubmed-meshheading:9538224-Animals,
pubmed-meshheading:9538224-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:9538224-Cell Differentiation,
pubmed-meshheading:9538224-Cell Division,
pubmed-meshheading:9538224-Epidermal Growth Factor,
pubmed-meshheading:9538224-Male,
pubmed-meshheading:9538224-Mice,
pubmed-meshheading:9538224-Nerve Growth Factors,
pubmed-meshheading:9538224-Neurites,
pubmed-meshheading:9538224-Neurons,
pubmed-meshheading:9538224-PC12 Cells,
pubmed-meshheading:9538224-Phosphorylation,
pubmed-meshheading:9538224-Proto-Oncogene Proteins,
pubmed-meshheading:9538224-Pyrimidines,
pubmed-meshheading:9538224-Rats,
pubmed-meshheading:9538224-Receptor, Epidermal Growth Factor,
pubmed-meshheading:9538224-Receptor, trkA,
pubmed-meshheading:9538224-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:9538224-Receptors, Nerve Growth Factor,
pubmed-meshheading:9538224-Signal Transduction,
pubmed-meshheading:9538224-Tyrosine
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pubmed:year |
1998
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pubmed:articleTitle |
MS-430, a synthetic pyrimidine derivative, influences the intracellular signal transduction pathway leading to neuronal differentiation of PC12h cells.
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pubmed:affiliation |
Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565, Japan. ikeuchi@protein.osaka-u.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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