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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1998-8-18
|
| pubmed:databankReference | |
| pubmed:abstractText |
Recently, we found a novel murine cell-surface glycoprotein, designated as p91, expressed mainly in myeloid cells such as macrophages and mast cells. The molecule has six immunoglobulin-like extracellular domains, a transmembrane segment, and a cytoplasmic tail containing four immunoreceptor tyrosine-based inhibition motif (ITIM) or ITIM-like sequences, resembling the structural features of human killer-cell inhibitory receptors (KIR). Here we show that p91 comprises a polymorphic gene family, harboring one potent inhibitory-type p91 and at least two other p91 genes. Tyrosine-phosphorylated, but not nonphosphorylated, synthetic peptides matching the third ITIM and the fourth ITIM-like sequences, respectively, found in the cytoplasmic portion of p91A, the sole inhibitory-type p91, were associated with the tyrosine phosphatases, SHP-1 and SHP-2. In addition, the phosphotyrosyl peptide matching the third ITIM sequence also bound the inositol 5-phosphatase, SHIP. These results support the notion that p91A may function as an inhibitory cell-surface molecule against cell activation. The p91 genes were shown to be clustered in the proximal region of mouse chromosome 7, a syntenic position of human chromosome 19 where the genes for the KIR family are found. A human cDNA clone cross-hybridizing to a murine p91 probe was isolated from a human spleen cDNA library, and was found to code for a molecule quite similar to members of the immunoglobulin-like transcript (or ILT) family. The gene was found to be located on human chromosome 19q13.3-13.4. These results establish the existence of a novel set of potent regulatory receptors in mouse and man, similar but different from the KIR family.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Pira1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Pirb protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, KIR
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0021-924X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
123
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
358-68
|
| pubmed:dateRevised |
2007-12-19
|
| pubmed:meshHeading |
pubmed-meshheading:9538215-Amino Acid Sequence,
pubmed-meshheading:9538215-Animals,
pubmed-meshheading:9538215-Cells, Cultured,
pubmed-meshheading:9538215-Chromosome Mapping,
pubmed-meshheading:9538215-Chromosomes, Human, Pair 19,
pubmed-meshheading:9538215-Cloning, Molecular,
pubmed-meshheading:9538215-Cytoplasm,
pubmed-meshheading:9538215-DNA, Complementary,
pubmed-meshheading:9538215-Humans,
pubmed-meshheading:9538215-Macrophages, Peritoneal,
pubmed-meshheading:9538215-Mice,
pubmed-meshheading:9538215-Mice, Inbred BALB C,
pubmed-meshheading:9538215-Mice, Inbred C57BL,
pubmed-meshheading:9538215-Mice, Inbred Strains,
pubmed-meshheading:9538215-Molecular Sequence Data,
pubmed-meshheading:9538215-Multigene Family,
pubmed-meshheading:9538215-RNA, Messenger,
pubmed-meshheading:9538215-Receptors, Immunologic,
pubmed-meshheading:9538215-Receptors, KIR,
pubmed-meshheading:9538215-Sequence Alignment,
pubmed-meshheading:9538215-Sequence Homology, Amino Acid
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Genomic structures and chromosomal location of p91, a novel murine regulatory receptor family.
|
| pubmed:affiliation |
Department of Biotechnology, Faculty of Engineering Okayama University, Tsushima-Naka.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|