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rdf:type |
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lifeskim:mentions |
|
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pubmed:issue |
7
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pubmed:dateCreated |
1998-4-16
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pubmed:abstractText |
Tumor cells that express a fusion gene comprised of Escherichia coli cytosine deaminase (CD) and herpes simplex virus type 1 thymidine kinase (TK) sequences exhibit activation of and subsequent killing by the normally innocuous prodrugs 5-fluorocytosine and ganciclovir (Rogulski et al., Hum. Gene Ther., 8: 73-85, 1997). To target localized expression of this therapeutic gene, we have constructed a recombinant adenovirus containing the CD-TK fusion gene under the control of a human inducible heat shock protein 70 promotional sequence. Strong expression of the fusion gene product was induced by heating at 41 degrees C for 1 h. Expression levels obtained were dependent on the multiplicity of infection used and the incubation time after heat shock. Heat-induced expression of the CD-TK protein significantly reduced the survival of PC-3 cells in the presence of both 5-fluorocytosine and ganciclovir. These studies represent a novel form of gene therapy for the transduction and regulation of a double suicide gene in tumor cells and may provide a unique application for hyperthermia in cancer therapy.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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|
pubmed:issn |
0008-5472
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
1
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|
pubmed:volume |
58
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1358-62
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9537229-Adenoviridae,
pubmed-meshheading:9537229-Antimetabolites,
pubmed-meshheading:9537229-Antiviral Agents,
pubmed-meshheading:9537229-Cytosine Deaminase,
pubmed-meshheading:9537229-Enzyme Activation,
pubmed-meshheading:9537229-Escherichia coli,
pubmed-meshheading:9537229-Flucytosine,
pubmed-meshheading:9537229-Ganciclovir,
pubmed-meshheading:9537229-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:9537229-Gene Therapy,
pubmed-meshheading:9537229-Gene Transfer Techniques,
pubmed-meshheading:9537229-Herpesvirus 1, Human,
pubmed-meshheading:9537229-Hot Temperature,
pubmed-meshheading:9537229-Humans,
pubmed-meshheading:9537229-Male,
pubmed-meshheading:9537229-Nucleoside Deaminases,
pubmed-meshheading:9537229-Prostatic Neoplasms,
pubmed-meshheading:9537229-Recombinant Fusion Proteins,
pubmed-meshheading:9537229-Thymidine Kinase
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pubmed:year |
1998
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|
pubmed:articleTitle |
Adenoviral-mediated transfer of a heat-inducible double suicide gene into prostate carcinoma cells.
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pubmed:affiliation |
Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, Michigan 48073-6769, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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