Linked Life Data

9535884

Source:http://linkedlifedata.com/resource/pubmed/id/9535884

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
1998-5-14
pubmed:abstractText
The type-3 inositol 1,4,5-trisphosphate (InsP3) receptor is the major isoform expressed in 16HBE14o- cells from bronchial mucosa, representing 93% at the mRNA level as determined by ratio reverse transcription-polymerase chain reaction and about 81% at the protein level as determined with isoform-specific antibodies (Sienaert, I., Huyghe, S., Parys, J. B., Malfait, M., Kunzelmann, K., De Smedt, H., Verleden, G. M., and Missiaen, L., Pflügers Arch. Eur. Y. Physiol., in press). The present 45Ca2+ efflux experiments indicate that these InsP3 receptors were 3 times less sensitive to InsP3 and 11 times less sensitive to ATP than those in A7r5 cells, where the type-1 InsP3 receptor is the main isoform. ATP did not increase the cooperativity of the InsP3-induced Ca2+ release in 16HBE14o- cells, in contrast to its effect in A7r5 cells. The sulfhydryl reagent thimerosal also did not stimulate InsP3-induced Ca2+ release in 16HBE14o- cells, again in contrast to its effect in A7r5 cells. Adenophostin A was more potent than InsP3 in stimulating the release in both cell types. The biphasic activation of the InsP3 receptor by cytosolic Ca2+ occurred in both cell types. We conclude that Ca2+ release mediated by the type-3 InsP3 receptor mainly differs from that mediated by the type-1 InsP3 receptor by its lack of stimulation by sulfhydryl oxidation and its lower ATP and InsP3 sensitivity. The predominant expression of the type-3 InsP3 receptor in the bronchial mucosa may be part of a mechanism coping with oxidative stress in that tissue.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
273
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8983-6
pubmed:dateRevised
2007-7-18
pubmed:meshHeading
pubmed-meshheading:9535884-Adenosine, pubmed-meshheading:9535884-Adenosine Triphosphate, pubmed-meshheading:9535884-Animals, pubmed-meshheading:9535884-Aorta, pubmed-meshheading:9535884-Bronchi, pubmed-meshheading:9535884-Calcium, pubmed-meshheading:9535884-Calcium Channels, pubmed-meshheading:9535884-Cell Line, pubmed-meshheading:9535884-Cell Membrane Permeability, pubmed-meshheading:9535884-Cytosol, pubmed-meshheading:9535884-Inositol 1,4,5-Trisphosphate, pubmed-meshheading:9535884-Inositol 1,4,5-Trisphosphate Receptors, pubmed-meshheading:9535884-Kinetics, pubmed-meshheading:9535884-Mucous Membrane, pubmed-meshheading:9535884-Polymerase Chain Reaction, pubmed-meshheading:9535884-RNA, Messenger, pubmed-meshheading:9535884-Rats, pubmed-meshheading:9535884-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:9535884-Thimerosal, pubmed-meshheading:9535884-Transcription, Genetic
pubmed:year
1998
pubmed:articleTitle
Functional properties of the type-3 InsP3 receptor in 16HBE14o- bronchial mucosal cells.
pubmed:affiliation
Laboratorium voor Fysiologie, K. U. Leuven Campus Gasthuisberg, Herestraat 49, B-3000 Leuven, Belgium. Ludwig.Missiaen@med.kuleuven.ac.be
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't