Linked Life Data

9533862

Source:http://linkedlifedata.com/resource/pubmed/id/9533862

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1998-6-30
pubmed:abstractText
Lens epithelial cells from many species contain inwardly rectifying K+ channels. The channels are highly selective for K+ over Na+. They have a conductance of 27-30 pS in symmetrical 150 mM K+ solutions. The conductance to inwardly flowing current depends on the external [K+], being 1/2 maximal at about 50 mM and maximal by 110-150 mM. The amino acid sequences from lens epithelium (eight different species) show at least 98% sequence homology to each other and to the potassium channel known as IRK1 (Kir 2.1). Cloned channels from chick, rabbit, and human lens epithelium all make functional channels when their cDNA is transfected into HEK-293 or tsA-201 cells. Human lens inward rectifiers when engineered as fusion proteins with green fluorescent protein (GFP) also make functional channels. In addition, their localization in the membrane and in intracellular organelles can be demonstrated by fluorescence microscopy.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0014-4835
pubmed:author
pubmed:copyrightInfo
Copyright 1998 Academic Press Limited.
pubmed:issnType
Print
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
347-59
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Inwardly rectifying potassium channels in lens epithelium are from the IRK1 (Kir 2.1) family.
pubmed:affiliation
Department of Physiology, May Foundation, Rochester, MN 55905, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't