| pubmed-article:9533765 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9533765 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:9533765 | lifeskim:mentions | umls-concept:C0022984 | lld:lifeskim |
| pubmed-article:9533765 | lifeskim:mentions | umls-concept:C0014912 | lld:lifeskim |
| pubmed-article:9533765 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:9533765 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:9533765 | pubmed:dateCreated | 1998-4-15 | lld:pubmed |
| pubmed-article:9533765 | pubmed:abstractText | We have investigated the effects of laminin, on the plasminogen-activator system of MCF-7 breast-carcinoma cells. MCF-7 cells were incubated on plastic or laminin-coated wells, and medium and cell lysate aliquots were assayed for tissue-type (tPA) and urokinase-type plasminogen activator (uPA) by a chromogenic assay in combination with anti-uPA antibodies. Cells cultured on laminin displayed a 5-fold increase in tPA activity and a 2-fold decrease in uPA activity relative to cells on plastic. These effects could be mimicked by laminin fragment P1 but not by collagen I or fibronectin. tPA activity of cells treated with estradiol (10 nM) was 3-fold higher, that of cells on laminin treated with estradiol was 15-fold higher, than that of control. Northern-blot analysis showed that tPA mRNA levels were up-regulated by estradiol and laminin, whereas PAI-1 mRNA levels were down-regulated by laminin and not affected by E2. Concomitant treatment with laminin and estradiol, decreased PAI-1 mRNA and increased tPA mRNA levels, accounting for the synergistic increase in tPA activity. Laminin exerted only a modest (approx. 2-fold) inhibitory effect on uPA mRNA levels. In the breast-carcinoma cell line MDA-MB-231, down-regulation of PAI-1 and uPA mRNA by laminin was not observed. Adhesion assays indicated that alpha2beta1 is the predominant receptor for laminin in MCF-7 cells. MDA-MB-231 cells expressed alpha2 (54%) but this integrin is not used as a laminin receptor. These results support a role for alpha2beta1 in mediating interactions of MCF-7 with LN. | lld:pubmed |
| pubmed-article:9533765 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9533765 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9533765 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9533765 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9533765 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9533765 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9533765 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9533765 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9533765 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9533765 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9533765 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:9533765 | pubmed:issn | 0020-7136 | lld:pubmed |
| pubmed-article:9533765 | pubmed:author | pubmed-author:BrentaniM MMM | lld:pubmed |
| pubmed-article:9533765 | pubmed:author | pubmed-author:SonoharaSS | lld:pubmed |
| pubmed-article:9533765 | pubmed:author | pubmed-author:Mira-y-LopezR... | lld:pubmed |
| pubmed-article:9533765 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9533765 | pubmed:day | 30 | lld:pubmed |
| pubmed-article:9533765 | pubmed:volume | 76 | lld:pubmed |
| pubmed-article:9533765 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9533765 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9533765 | pubmed:pagination | 77-85 | lld:pubmed |
| pubmed-article:9533765 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:9533765 | pubmed:meshHeading | pubmed-meshheading:9533765-... | lld:pubmed |
| pubmed-article:9533765 | pubmed:meshHeading | pubmed-meshheading:9533765-... | lld:pubmed |
| pubmed-article:9533765 | pubmed:meshHeading | pubmed-meshheading:9533765-... | lld:pubmed |
| pubmed-article:9533765 | pubmed:meshHeading | pubmed-meshheading:9533765-... | lld:pubmed |
| pubmed-article:9533765 | pubmed:meshHeading | pubmed-meshheading:9533765-... | lld:pubmed |
| pubmed-article:9533765 | pubmed:meshHeading | pubmed-meshheading:9533765-... | lld:pubmed |
| pubmed-article:9533765 | pubmed:meshHeading | pubmed-meshheading:9533765-... | lld:pubmed |
| pubmed-article:9533765 | pubmed:meshHeading | pubmed-meshheading:9533765-... | lld:pubmed |
| pubmed-article:9533765 | pubmed:meshHeading | pubmed-meshheading:9533765-... | lld:pubmed |
| pubmed-article:9533765 | pubmed:meshHeading | pubmed-meshheading:9533765-... | lld:pubmed |
| pubmed-article:9533765 | pubmed:meshHeading | pubmed-meshheading:9533765-... | lld:pubmed |
| pubmed-article:9533765 | pubmed:meshHeading | pubmed-meshheading:9533765-... | lld:pubmed |
| pubmed-article:9533765 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9533765 | pubmed:articleTitle | Laminin and estradiol regulation of the plasminogen-activator system in MCF-7 breast-carcinoma cells. | lld:pubmed |
| pubmed-article:9533765 | pubmed:affiliation | Departamento de Radiologia de Faculdade de Medicina da USP, São Paulo, Brazil. | lld:pubmed |
| pubmed-article:9533765 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9533765 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
