Linked Life Data

9533551

Source:http://linkedlifedata.com/resource/pubmed/id/9533551

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1998-5-28
pubmed:abstractText
A novel tumor suppressor gene, PTEN, which encodes a dual-specificity protein phosphatase, has recently been identified on chromosome 10q23. We have previously shown that both alleles of this gene are inactivated in three of four prostate cancer cell lines tested. To evaluate the role of inactivation of this gene in primary stage B prostate cancers, 60 cases were analyzed using Southern blotting with PTEN probes and microsatellites on 10q23. Eight of 60 cases had homozygous deletions by Southern blotting. In three of these cases, homozygous deletion was confirmed by apparent retention of heterozygosity at PTEN with loss of heterozygosity at telomeric and centromeric loci. In the remaining five cases, microsatellite analysis was consistent with homozygous deletion. Loss of heterozygosity at PTEN was found in only two cases both by microsatellite analysis and quantitative Southern blotting. No small mutations within PTEN exons were found in any tumors exhibiting alterations on 10q23. Thus, inactivation of the PTEN gene by homozygous deletion occurs in approximately 10-15% of primary stage B prostate carcinomas.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1078-0432
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
811-5
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:9533551-Adenocarcinoma, pubmed-meshheading:9533551-Blotting, Southern, pubmed-meshheading:9533551-Chromosome Mapping, pubmed-meshheading:9533551-Chromosomes, Human, Pair 10, pubmed-meshheading:9533551-Gene Deletion, pubmed-meshheading:9533551-Genes, Tumor Suppressor, pubmed-meshheading:9533551-Homozygote, pubmed-meshheading:9533551-Humans, pubmed-meshheading:9533551-Male, pubmed-meshheading:9533551-Microsatellite Repeats, pubmed-meshheading:9533551-Neoplasm Staging, pubmed-meshheading:9533551-PTEN Phosphohydrolase, pubmed-meshheading:9533551-Phosphoric Monoester Hydrolases, pubmed-meshheading:9533551-Polymerase Chain Reaction, pubmed-meshheading:9533551-Prostatic Neoplasms, pubmed-meshheading:9533551-Protein Tyrosine Phosphatases, pubmed-meshheading:9533551-Tumor Suppressor Proteins
pubmed:year
1998
pubmed:articleTitle
Homozygous deletion of the PTEN tumor suppressor gene in a subset of prostate adenocarcinomas.
pubmed:affiliation
Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't