Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1998-4-21
|
| pubmed:abstractText |
IL-12 (or LPS) priming and subsequent challenge by LPS produces the generalized Shwartzman reaction. IFN-gamma induced by IL-12 is a crucial cytokine in the priming phase. In vivo depletion of both NK cells and NK1+ alphabeta T cells of mice by anti-NK1.1 Ab greatly reduced the elevation of serum IFN-gamma induced by IL-12 and significantly reduced mortality after subsequent injection of LPS, whereas depletion of NK cells alone by anti-asialo GM1 Ab only partially decreased serum IFN-gamma, and lethality was not changed. Cell sorting and culture experiments confirmed that liver NK1+ alphabeta T cells of IL-12-injected mice produced greater amounts of IFN-gamma than did liver NK cells. MHC class I-deficient mice of C57BL/6 background, which lack a majority of NK1+ alphabeta T cells, produced low amounts of IFN-gamma by IL-12; no mortality was observed after the LPS challenge. However, production of TNF-alpha in the second phase (after LPS challenge) was not inhibited by depletion of NK cells alone or both subsets. IL-12 and subsequent LPS challenge activated NK1+ alphabeta T cells in the liver and induced strong cytotoxicity of these cells not only against tumor cells (including Fas-negative tumors) but also against a syngeneic hepatocyte cell line. Our findings show that IFN-gamma produced by NK1+ alphabeta T cells is essential for the IL-12 priming of the Shwartzman reaction, and the autoreactivity of NK1+ alphabeta T cells in the liver is involved in the hepatic disorders that are sometimes caused by IL-12, LPS, or the generalized Shwartzman reaction.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD1,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
160
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3522-7
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9531314-Adjuvants, Immunologic,
pubmed-meshheading:9531314-Animals,
pubmed-meshheading:9531314-Antigens, CD1,
pubmed-meshheading:9531314-Cell Movement,
pubmed-meshheading:9531314-Cytotoxicity, Immunologic,
pubmed-meshheading:9531314-Injections, Intraperitoneal,
pubmed-meshheading:9531314-Interferon-gamma,
pubmed-meshheading:9531314-Interleukin-12,
pubmed-meshheading:9531314-Leukocytes, Mononuclear,
pubmed-meshheading:9531314-Lipopolysaccharides,
pubmed-meshheading:9531314-Liver,
pubmed-meshheading:9531314-Lymphocyte Activation,
pubmed-meshheading:9531314-Lymphocyte Depletion,
pubmed-meshheading:9531314-Male,
pubmed-meshheading:9531314-Mice,
pubmed-meshheading:9531314-Mice, Inbred C57BL,
pubmed-meshheading:9531314-Mice, Mutant Strains,
pubmed-meshheading:9531314-Mice, Nude,
pubmed-meshheading:9531314-Shwartzman Phenomenon,
pubmed-meshheading:9531314-T-Lymphocyte Subsets,
pubmed-meshheading:9531314-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:9531314-Tumor Necrosis Factor-alpha
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Involvement of NK1+ T cells and their IFN-gamma production in the generalized Shwartzman reaction.
|
| pubmed:affiliation |
Department of Microbiology, Tohoku University School of Dentistry, Sendai, Japan.
|
| pubmed:publicationType |
Journal Article
|